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Strain stability of plasmid-containing recombinant organisms is clearly important for industrial applications. Stability is normally assessed by methods such as selective colony forming units or by simply measuring the recombinant product. These methods are typically performed off-line, are time-consuming, and do not give detailed information on the changes in the metabolism. In the current work,
BACKGROUND The sperm chromatin structure assay (SCSA) parameter DNA fragmentation index (DFI) has been shown to predict in vivo and in vitro fertility. So far most SCSA studies have been based on SCSA analysis performed on neat semen. The aim of this study is to assess whether SCSA analysis of sperm prepared by density gradient centrifugation (DGC) could add more information in regard to the predi
Departing from three metaphors—the body as gift, resource, and commodity—the book explores the contemporary exchange of organs, tissues, and cells. Although the gift is the sanctioned metaphor for donating parts of the body, the underlying perspective from the side of states, authorities, and the medical establishment often seems to be that the body shall be understood as a resource. But medicine,
Objective: This multiple case study investigated support and process in the Individual Placement and Support (IPS) approach from individual client, longitudinal and Person-Environment-Occupation (PEO) model perspectives. Participants: Five IPS-participants, or cases, with severe mental illness (SMI) who worked a minimum of 4 hours a week entered the study. Methods: A multiple data collectio
Attempted suicide is a risk factor for future suicidal behaviour, but understanding suicidality from the perspective of people who have experienced attempted suicide is limited. The aim of the study was to explore the lived experience of being suicidal and having made a suicide attempt, in order to identify possible implications for health care professionals. Semi-structured individual interviews
Popular Abstract in English In the past ten years, homogeneous charge compression ignition (HCCI) combustion has been one of the subjects receiving great research interests in the combustion research community and the engine industry. This is because HCCI combustion processes have the potential of not only capable providing high efficiency like diesel engine combustion, but also producing much lowLarge Eddy Simulation (LES) modeling is developed to study homogeneous charge compression ignition (HCCI) combustion under different engine operation and mixture conditions, including real engine configurations and generic test cases. A previously developed HCCI model has been validated by comparing the simulation results with engine experiments. A new spark-assisted HCCI (SACI) combustion model h
Today, there is considerable interest in integrated forest biorefineries which, besides pulp, produce high-value-added products, such as, polymers and carbon fibres. Lignin is the major non-cellulosic constituent of wood. It is a complex, amorphous highly crosslinked polyphenolic. Lignosulphonate in spent liquor in sulphite mills has long been isolated using ultra filtration. Generally, precipitat
A new way of preparing catalytically active wire meshes through a thermal-spray technique is described. A metal substrate (e.g. Kanthal AF) was plasma-sprayed with a composite ceramic/polymer-powder. The polymer content of the sprayed layer was burnt off whereupon a well-defined macro-porosity was created. By treating the so obtained material with an alumina-sol the specific surface area could be
Background: Previous research has focused exclusively on weight loss or weight maintenance following weight loss, i.e. secondary weight maintenance (SWM). The long-term results of SWM have been modest, suggesting that preventing initial weight gain among normal weight or overweight individuals, i.e. primary weight maintenance (PWM), may be more successful. The aim of this study was to compare the
Development of artificial neural network (ANN) models using real plant data for the prediction of fresh steam properties from a brown coal-fired boiler of a Slovenian power plant is reported. Input parameters for this prediction were selected from a large number of available parameters. Initial selection was made on a basis of expert knowledge and previous experience. However, the final set of inp
Emissions and fuel economy are evaluated for two types of biofuels, namely 2-phenyl ethanol and cyclohexaneethanol. Both are derived from lignin, a form of lignocellulosic biomass. The former and latter oxygenates have an aromatic and aliphatic (i.e. saturated) ring structure, respectively. Two parametric engine sweeps were performed. First, non-EGR operation by means of varying the load via injec
Context The relationship of platelet function testing measurements with outcomes in patients with acute coronary syndromes (ACS) initially managed medically without revascularization is unknown. Objective To characterize the differences and evaluate clinical outcomes associated with platelet reactivity among patients with ACS treated with clopidogrel or prasugrel. Design, Setting, and Patients Pat
Refillable polyester bottles, for example polyethylene terephthalate (PET) bottles, are known to interact with chemicals. Aroma compounds from a product can be absorbed by the bottle material, remain after washing and remigrate when the bottle is reused, resulting in off-flavour of the new product. A certified reference material has recently been approved with which the chemical and sensory inertn
Disentangling the individual and interactive effects of multiple stressors on microbial communities is a key challenge to our understanding and management of ecosystems. Advances in molecular techniques allow studying microbial communities in situ and with high taxonomic resolution. However, the taxonomic level which provides the best trade-off between our ability to detect multiple-stressor effec
Upper extremity blocks are useful as both sole anaesthesia and/or a supplement to general anaesthesia and they further provide effective postoperative analgesia, reducing the need for opioid analgesics. There is without doubt a renewed interest among anaesthesiologists in the interscalene, supraclavicular, infraclavicular, and axillary plexus blocks with the increasing use of ultrasound guidance.
The results of a search for the direct pair production of top squarks, the supersymmetric partner of the top quark, in final states with one isolated electron or muon, several energetic jets, and missing transverse momentum are reported. The analysis also targets spin-0 mediator models, where the mediator decays into a pair of dark-matter particles and is produced in association with a pair of top
To sample information optimally, sensory systems must adapt to the ecological demands of each animal species. These adaptations can occur peripherally, in the anatomical structures of sensory organs and their receptors; and centrally, as higher-order neural processing in the brain. While a rich body of investigations has focused on peripheral adaptations, our understanding is sparse when it comes
We investigated the prevalence of postnatal depressive mood in a national Swedish sample, together with factors associated with depressed mood postpartum only as opposed to having depressed mood both in early pregnancy and postpartum. Swedish-speaking women booked for antenatal care during a chosen period of three recruitment weeks were invited and 3293 (72%) agreed to participate in the study. Of
