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Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the pe
To better understand how deformable and non-spherical particles behave in sorting devices based on deterministic lateral displacement we generate models of biological particles with tunable size, shape and mechanical properties using stop-flow lithography and we explore how these parameters play a role in our separation devices. Hollow and solid cylinders are compared with respect to their deforma
We present a simple and rapid microfluidic device capable of extracting and concentrating the parasite causing the fatal disease sleeping sickness (SS) from blood. The device is based on deterministic lateral displacement (DLD) and constructed with a single inlet with flow induced by an ordinary syringe. The simplicity is crucial as the device is intended for use in the resource depraved areas whe
We present a simple and inexpensive device capable of extracting and concentrating the parasite causing sleeping sickness from blood. The device is aimed at being used in rural resource depraved areas where the disease is endemic; therefore simplicity is of paramount importance. The device is based on deterministic lateral displacement with a single inlet and flow induced by a syringe. Through an
This paper draws upon an extensive transdisciplinary scenario development in the context of the stakeholder oriented preparation of the climate protection plan of the German federal state North Rhine-Westphalia, which is home to the most important heavy industry cluster in Europe. In that context we developed differentiated bottom up climate change mitigation strategies and scenarios for the major
We present an advanced approach for single-cell analysis of circulating tumor cells (CTCs) by integrating an acoustofluidic chip for sample preparation, including simultaneous acoustophoretic separation and enrichment of the separated cells, onto an electroactive microwell array (EMA) for single-cell arraying. The integrated device shows highly efficient cell recovery of target cells (82.5%) and d
We have created devices that are extremely simple to fabricate, function without pumps, are reusable and capable of dealing with very large differences in particle sizes without clogging. Our new approach to microfluidic particle sorting is precise enough for applications in blood fractionation, cancer cell sorting, parasite detection and sample decomplexing in general. We achieve this using Deter
High-power operation of conventional GaN-based light-emitting diodes (LEDs) is severely limited by current crowding, which increases the bias voltage of the LED, concentrates light emission close to the p-type contact edge, and aggravates the efficiency droop. Fabricating LEDs on thick n-GaN substrates alleviates current crowding but requires the use of expensive bulk GaN substrates and fairly lar
The precise separation of nanoscale particles has proven challenging due to diffusion and the need to use nanoscale devices. We show the separation of particles in the 100 nm size range in Deterministic Lateral Displacement (DLD) devices with feature sizes in the 10 µm size range. We achieve this using Dielectrophoretic (DEP) forces, generated between the metal coated posts that act as active elec
Recent research has identified the grasslands of the Sahel as a potential carbon sink, but regional carbon stock accounting in such biomes is undermined by the poorly recognized role they play in the carbon cycle as well as lack of data. In this paper, we demonstrate the feasibility of coupling the satellite data-driven light use efficiency model (Lund University Light Use Efficiency Model - LULUE
Research on human emotions towards large carnivores is often based on self-reported emotions. This study aimed to investigate physiological and behavioral responses to feared animals among people who say that they are fearful or not fearful of brown bear and wolf. Participants were recruited to be bear fearful only (n=8), fearful of both bear and wolves (n=15), or fearful of neither carnivore (n=1
As our society becomes more and more dependent on IT systems, failures of these systems can harm more and more people and organizations both public and private. Diligently performing risk and hazard analysis helps to minimize the societal harms of IT system failures. In this paper we present experiences gained by applying the System Theoretic Process Analysis (STPA) method for hazard analysis on a
Many psychiatric illnesses are associated with early mortality and with an increased risk of developing physical diseases that are more typically seen in the elderly. Moreover, certain psychiatric illnesses may be associated with accelerated cellular aging, evidenced by shortened leukocyte telomere length (LTL), which could underlie this association. Shortened LTL reflects a cell's mitotic history
The paper examines the reception of and revisions made to the teachings of the Italian astrologer-monk Placido de Titi (1603 – 1668) in Protestant England following the decline of astrology during the 18th century, particularly as found in the writings of John Worsdale (1766 – c. 1826), whose role in the emergence of modern astrology is to a certain extent reappraised, and of his contemporary crit
We present an estimate for the convergence rate of the Dirichlet-Neumann iteration for the discretized unsteady transmission problem. Specifically, we consider the coupling of two heat equations on two identical squared domains. The Laplacian is discretized by second order central finite differences and the implicit Euler method is used for the time discretization. For the semidiscrete case, Hensh
Economies in Transition (EiTs) exemplified by countries such as Poland, Ukraine, Belarus, Moldova etc. face many socio-economic and environmental challenges – energy security, high unemployment, poor transportation and municipal service infrastructure, and rural depopulation are common themes. Agricultural biofuel production offers a number of socio-economic co-benefits that can contribute to the
We present a FrameNet-based semantic role labeling system for Swedish text. As training data for the system, we used an annotated corpus that we produced by transferring FrameNet annotation from the English side to the Swedish side in a parallel corpus. In addition, we describe two frame element bracketing algorithms that are suitable when no robust constituent parsers are available. We evaluated
Corrosion of cables can result in brittle and unexpected failures and represents a critical concern for prestressed concrete bridges with post-tensioned (PT) tendons. In these structures, corrosion is typically facilitated by inadequate grout injection and/or the use of poor-quality mortar during construction. Such deficiencies create favorable conditions for corrosion initiation and propagation.
