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This study describes the design of degenerate primers and their use for synthesis of full-length avian influenza A neuramindase (NA). Each reaction was performed using either two forward primers and one reverse primer, or one forward primer and one reverse primer. Both primer combinations had comparable amplification efficiencies for all NA subtypes (1-9). A total of 115 virus strains, including b

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Baker’s yeast, Saccharomyces cerevisiae, is a promising cell factory for the sustainable utilization of renewable resources for the formation of products with commercial value. Among these, poly-3-D-hydroxybutyrate (PHB) is an extensively studied biopolymer naturally accumulated in some bacteria and archaea species through the formation of carbon granules. Its bio-based origin, biodegradability an

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Correct phenotypic interpretation of variants of unknown significance for cancer-associated genes is a diagnostic challenge as genetic screenings gain in popularity in the next-generation sequencing era. The Critical Assessment of Genome Interpretation (CAGI) experiment aims to test and define the state of the art of genotype-phenotype interpretation. Here, we present the assessment of the CAGI p1

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We consider designing demodulators for linear vector channels that use reduced-size trellis descriptions for the received signal. We assume an iterative receiver and use interference cancellation (IC) with soft-information provided by an outer decoder to mitigate the signal part that is not covered by a reduced-size trellis description. In order to reach a trellis description, a linear filter is a

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The Environmental Determinants of Diabetes in the Young (TEDDY) is an international study aiming to investigate associations between dietary and other environmental factors and the risk of developing islet autoimmunity and type 1 diabetes. Dietary intake was assessed using a 24-h recall and repeated 3-day food records and analyzed using country-specific food composition databases (FCDBs) in Finlan

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β-1,4-Galactosyltransferase 7 (β4GalT7) is a key enzyme in the biosynthesis of glycosaminoglycan (GAG) chains. Natural and synthetic xylosides can be used to both inhibit and prime GAG synthesis by acting as inhibitors or substrates for β4GalT7. In this report, we exploit hydroxylated oxanes as deoxygenated xyloside analogs to clarify the minimum protein-ligand interactions required for galactosyl

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Objective: The European System for Cardiac Operative Risk Evaluation (EuroSCORE) is used to identify patients at high risk for aortic valve replacement (AVR) in whom alternative procedures, such as trans-catheter aortic valve implantation (TAVI), may be appropriate. The aim of the present study was to calibrate and validate the EuroSCORE for different cardiac surgery procedures to improve patient

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This study describes and validates a new method for automatic segmentation of left ventricular mass (LVM) in myocardial perfusion SPECT (MPS) images. This is important for estimating the size of a perfusion defect as percentage of the left ventricle. METHODS: A total of 101 patients with known or suspected coronary artery disease underwent both rest and stress MPS and MRI. A new automated algorith

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The natural radiative lifetime of the 3s 4p 1P1 level of neutral magnesium was measured by pulsed level-crossing spectroscopy at the short excitation wavelength of 202.6 nm. We obtained tau = 13.4(5) ns for this state, strongly deviating from a previous beam-foil result, but in good agreement with a multi-configuration Hartree-Fock calculation. A discussion of the applicability of the pulsed Hanle

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Scand J Caring Sci; 2011 Online self-help tools for the relatives of persons with depression - a feasibility study Background: The Internet's potential as health care tool should be explored. Aim: One objective was to determine the feasibility of constructing a digitally based tool through an iterative design process in cooperation with potential users. The tool's purpose is to alleviate hardships

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We present a portable system for non-invasive, simultaneous sensing of molecular oxygen (O-2) and water vapor (H2O) in the human paranasal cavities. The system is based on high-resolution tunable diode laser spectroscopy (TDLAS) and digital wavelength modulation spectroscopy (dWMS). Since optical interference and non-ideal tuning of the diode lasers render signal processing complex, we focus on Fo

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Two different mathematical methods of implementing a detailed kinetic soot model have been employed in this work. The theoretical description of the soot modeling employed in this work is divided into three parts. Initially a detailed kinetic soot model is described from a chemistry and physics perspective. The soot model is then elaborated into mathematical form, starting with the formulation of

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AIMS: To assess the Edinburgh Postnatal Depression Scale (EPDS), a self-administered questionnaire created to screen for symptoms of postpartum depression in the community, from an epidemiological and ethical perspective. Screening, as the practice of investigating apparently healthy individuals to detect unrecognised disease or its precursors, has interpretation problems and is complicated by del

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The design and features of frequency-selective wallpaper created for attachment onto regular walls in order to filter out signals operating at 5 GHz while at the same time allowing the desired radio communication services to propagate through such walls are presented. An analysis of the characteristics of the radio channel evaluated in a typical indoor environment, when considering either regular

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The cavitation phenomenon has today become a keystone for different areas of science and technology including various industrial processes and medicine. Recently increasing interest and much attention have been paid to study this phenomenon. The last two decades have witnessed the development of numerous devices for different important applications of considerable practical interest, and the rang

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The Lake Poopo basin, located on the Bolivian Altiplano, is extremely vulnerable to environmental degradation. The basin displays extreme spatial and temporal variations of water resources and rapidly decreasing water quality due to anthropogenic and natural pollution. The region’s population lives in extreme poverty, and the authorities’ efforts to manage the water resources efficiently have been