Filtyp
The incidence of allergy is steadily increasing, but the molecular mechanisms involved in the allergic immune response are still not fully understood. In particular, further investigations focusing on dendritic cells, which are central in orchestrating the immune response, are needed. The objective of this study was to investigate the ability of myeloid leukaemia-derived cell lines, such as KG-1,
An integrated cell cultivation and protein product separation process was developed using a new type of supermacroporous polyacrylamide gel, called cryogel (pAAm-cryogel) support matrix. Human fibrosarcoma HT1080 and human colon cancer HCT116 cell lines were used to secrete urokinase (an enzyme of immense therapeutic utility) into the culture medium. The secreted protein was isolated from the circ
The feasibility and scalability of extraction in detergent-based aqueous two-phase systems for the separation of proteins from culture broth is demonstrated. At the same time the large-scale production of a fusion protein and the influence of cultivation scale on the efficiency of separation were investigated. An amphiphilic fusion protein EGIcore-HFBI was chosen, consisting of the catalytic core
Phonological vowel reduction in Bulgarian is discussed, distinguishing between formal & informal speech & dialectal & standard forms. The Bulgarian vowel system is presented, with an evaluation of the articulatory models of Bulgarian vowel production, & a description of the orthographic representation of the "indeterminate vowel." Alternations between stressed & nonstressed vow
Previous studies have shown increased cardiovascular mortality as late side effects in Hodgkin lymphoma (HL) patients. This study identifies stratifying risk factors for surveillance and defines concepts for a clinical feasible and noninvasive prospective protocol for intervention of cardiovascular side effects. HL patients diagnosed between 1965 and 1995 (n = 6.946) and their first-degree relativ
In an accompanying paper (Am. J. Epidemiol. 2002;155:1066-73), the authors describe the design of a large multicenter study being carried out in three West African countries for investigation of the roles of environmental and host-related factors in the development of tuberculosis. In this paper, the authors review some evidence that host genetic factors play a role in susceptibility to tuberculos
The anticancer active complex cisplatin interacts preferentially with the common, G - C rich, wobble base pair region of both tRNA(Ala) and Mh(Ala) in a reaction that at pH 6.3 is rate limited by the acid hydrolysis of the metal complex.
Inhibitors of the enzyme dipeptidyl peptidase IV (DPP IV) are of increasing interest to both diabetologists and the pharmaceutical industry alike, as they may become established as the next member of the oral antidiabetic class of therapeutic agents, designed to lower blood glucose and, possibly, prevent the progressive impairment of glucose metabolism in patients with impaired glucose tolerance a
SoTL
The use of electron probes for in situ imaging of solution phase systems has been a long held objective, largely driven by the prospect of atomic resolution of molecular structural dynamics relevant to chemistry and biology. Here, we present a nanofluidic sample cell with active feedback to maintain stable flow conditions for pathlengths varying from 45 nm to several 100 nm, over a useable viewing
The electronic structure of strongly correlated systems is usually calculated by using an effective model Hamiltonian with a small number of states and an effective on-site interaction. The mode, however, neglects the frequency dependence of the interaction, which emerges as a result of dynamical screening processes not included in the model. The self-energy calculated in this kind of model within
Background: Women working in the public human service sector in 'overstrained' situations run the risk of musculoskeletal symptoms and long-term sick leave. In order to maintain the level of health and work ability and strengthen the potential resources for health, it is important that employees gain greater control over decisions and actions affecting their health - a process associated with the
We have measured the oscillator strength of the Ni I line at 6300.34Å, which is known to be blended with the forbidden line [O I]lambda6300 used for the determination of the oxygen abundance in coolstars. We also give wavelengths of the two isotopic line components of58Ni and 60Ni derived from the asymmetriclaboratory line profile. These two line components of Ni I have to be
Popular Abstract in Swedish Maligna mjukdelstumörer, så kallade mjukdelssarkom, utgör hos vuxna en ovanlig tumörform som uppkommer i kroppens stödjevävnader. Till dessa räknas muskler, fett, bindväv, slemsäckar samt kärl och nerver som försörjer dessa vävnader. Trots att stödjevävnaderna omfattar mer än hälften av den totala kroppsvikten, utgör mjukdelssarkomen mindre än 1 % av alla maligna tumöreIn adult soft tissue sarcoma (STS) of the extremities and trunk wall, improved prognostic factors are needed to identify patients at high-risk for metastasis. Various factors are included in the many prognostic systems currently in use and the prognostic value of immunohistochemical (IHC) expression of biological markers is unclear. The tissue-preserving, high throughput tissue microarray (TMA) te
We investigate the scattering and multiple scattering of a typical laser beam (lambda= 800 nm) in the intermediate scattering regime. The turbid media used in this work are homogeneous solutions of monodisperse polystyrene spheres in distilled water. The two-dimensional distribution of light intensity is recorded experimentally, and calculated via Monte Carlo simulation for both forward and side s
Experimental studies helped to compare methods for determining and calculating total heat resistance of clothing sets accepted by Russian and international standards. Findings are that difference in the total heat resistance values calculated on humans and on dummies in relatively still air and quiet conditions is caused by measurement technique applied. Significant difference in the total heat re
