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Mixed cultures of a heterotrophic nitrifier/aerobic denitrifier, Thiosphaera pantotropha, and an autotrophic nitrifier, Nitrosomonas europaea, were grown in chemostats under dual ammonia-and acetate limitation. Because of simultaneous nitrification and denitrification by T. pantotropha, the activity of the cultures was evaluated from nitrogen balances as complete as possible. Under most conditions
A general, unstructured mathematical model has been used to describe the behaviour of nutrient-limited growth of two bacteria in a continuous co-culture. The experimental system consisted of a two-membered mixed culture of the heterotrophic nitrifier/aerobic denitrifier, Thiosphaera pantotropha, and the autotrophic nitrifier, Nitrosomonas europaea, competing for ammonia in chemostat culture. The o
During substrate-limited growth, some strains of Thiosphaera pantotropha nitrify heterotrophically and denitrify aerobically. These processes are thought to be used as NADH overflow mechanisms, resulting from a restricted oxygen respiration capacity. In this study, the behavior of T. pantotropha regarding the NADH overflow in the presence of substantial concentrations of substrate was examined. Co
Pancreatic cancer is one of our most lethal malignancies. Despite substantial improvements in the survival rates for other major cancer forms, pancreatic cancer survival rates have remained relatively unchanged since the 1960s. Pancreatic cancer is usually detected at an advanced stage and most treatment regimens are ineffective, contributing to the poor overall prognosis. Herein, we review the cu
This work treats multi-pitch estimation, and in particular the common misclassification issue wherein the pitch at half of the true fundamental frequency, here referred to as a sub-octave, is chosen instead of the true pitch. Extending on current methods which use an extension of the Group LASSO for pitch estimation, this work introduces an adaptive total variation penalty, which both enforce grou
Disrupted high school experiences, including dropout, are educational consequences for many youth with histories of homelessness. Using an ecological resilience prediction model (ERPM) based on the literature on resilience in at-risk youth, the study followed 82 youth who were initially homeless for a 2-year period, to identify predictors of participating in school. Female sex and increased durati
In order to understand where and how the Swedish public access their medicines, especially prescribed medicines, an online survey was conducted as a pilot study during April and May 2016. Among a collection of 155 answers, the data shows that, although a majority of the respondents feel hesitated and negative towards shopping prescribed medicines online, a tendency is demonstrated that people woul
National incidence and prevalence estimates of homelessness in Canadian youth are unknown. However, a recent annual profile of shelter users in a large urban centre estimated that one in five consumers of emergency shelter services are youth. Adolescence is a period of vulnerability from developmental perspective. In their progression from childhood to adulthood, youth have multiple role transitio
The aim of this article is to analyze reported changes in outdoor mobility, increased/unchanged/decreased, for a sample of older people (>62 years) in two regions in Sweden, who have transitioned from a two-person to a single-person household during the two years since the study was conducted. The target group (N=162) consists of all people who had transitioned to a single-person household in a ra
Successful optimization of a given lead scaffold requires thorough binding-site mapping of the target protein particular in regions remote from the catalytic center where high conservation across protein families is given. We screened a 361-entry fragment library for binding to the aspartic protease endothiapepsin by crystallography. This enzyme is frequently used as surrogate for the design of re
Crystallographic screening of the binding of small organic compounds (termed fragments) to proteins is increasingly important for medicinal chemistry-oriented drug discovery. To enable such experiments in a widespread manner, an affordable 96-compound library has been assembled for fragment screening in both academia and industry. The library is selected from already existing protein-ligand struct
Today the identification of lead structures for drug development often starts from small fragment-like molecules raising the chances to find compounds that successfully pass clinical trials. At the heart of the screening for fragments binding to a specific target, crystallography delivers structural information essential for subsequent drug design. While it is common to search for bound ligands in
Fragment-based lead discovery (FBLD) has become a pillar in drug development. Typical applications of this method comprise at least two biophysical screens as prefilter and a follow-up crystallographic experiment on a subset of fragments. Clearly, structural information is pivotal in FBLD, but a key question is whether such a screening cascade strategy will retrieve the majority of fragment-bound
