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We previously reported that insulin stimulation of human platelets induces serine phosphorylation and activation of the cGMP-inhibited cAMP phosphodiesterase (cGI-PDE). Here, we describe methods to detect and partially purify an insulin-stimulated cGI-PDE kinase (cGI-PDE ISK) from lysates of platelets incubated with insulin. Incubation of human platelets with 10(-8) M insulin increased cGI-PDE ISK

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Popular Abstract in Swedish Bakgrund Denna studie är en undersökning av barns musikskapande med synthesizer och dator. I studien deltog nio barn i åttaårsåldern. Musik utgör en viktig del i barns och ungdomars vardag och den stora exponering för musik som sker i vårt samhälle innebär att ingen är någon musikalisk nybörjare. Det är väl känt att barn kan skapa musik på egen hand, mest är det dock bToday’s children live in a world where music in all its different forms has become a significant factor in their everyday life. From earlier research we know that young children are able to create music by singing and using musical instruments. The present study was undertaken with eight-year-old children in school year 2 in order to investigate the children’s composing and composition processes u

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Chloroplasts isolated from a plant species, Dimorphotheca pluvialis Moench (Asteraceae), grown under visible light without supplementary UV-B radiation, have poor grana development. The dominant components in the membrane organizations are doublets and triplets of stroma lamellae. However, such chloroplasts possess the capacity for electron transfer through the whole photosynthetic chain, based on

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BACKGROUND: Persistent organochlorine pollutants (POPs) may interfere with reproductive function but direct evidence in humans is very limited. METHODS: Fertility was examined in four regions with contrasting blood levels of POPs. Pregnant women and their partners in Warsaw (Poland), Kharkiv (Ukraine) and Greenland were consecutively enrolled during antenatal visits. Swedish fishermen and their sp

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Three isoforms of the major birch pollen allergen, Bet v, 1 from Betula verrucosa have been expressed as recombinant proteins in E. coli and purified. The immunochemical properties of recombinant isoforms (rBet v 1) differed on immunoblots when compared using Mabs and birch pollen allergic patients serum IgE. 2-D gel analysis showed that recombinant isoforms with different epitope structure can fo

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Abstract in Undetermined Not available

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A selective pressurized liquid extraction (SPLE) procedure capable of performing simultaneous extraction and clean-up has been demonstrated for multi-residue analysis of organochlorine pesticides (OCPs) in soil. The final method was performed at 100 degrees C for 3 x 10 min using acetone/n-heptane (1:1, v/v). Florisil was placed inside the extraction cell downstream the sample to remove interferin

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The development of inhibitory antibodies against coagulation factor VIII (FVIII) in patients with mild haemophilia A is uncommon. We describe here two families in which three or two members have developed inhibitors, suggesting a familial predisposition. The mutations found, in the A2 (Arg593Cys) and C1 domains (Tyr2105Cys), have been reported to give rise to inhibitor development in single indivi

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Measurements are presented of inclusive charm and beauty cross sections in e(+)p collisions at HERA for values of photon virtuality 12 <= Q(2) = 60 GeV2 and of the Bjorken scaling variable 0.0002 <= x <= 0.005. The fractions of events containing charm and beauty quarks are determined using a method based on the impact parameter, in the transverse plane, of tracks to the primary vertex, as measured

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Resultat fran undersökningen visar att personrisker (inklusive dödsrisken) vid brand i ett småhus är tämligen oberoende av husets konstruktion. Trähus och stenhus kan därför klassificeras som likvardiga. Egendomsskador påverkas förutom av husets innehåll och beskaffenhet även av husets utformning och konstruktion. En brännbar konstruktion, speciellt vindsbjälklag- och yttertakkonstruktion innebär

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We report an observation of substantial deviations in the photoelectron angular distribution for photoionization of atoms in free Xe clusters compared to the case of photoionization of free atoms. The cross section, however, seems not to vary between the cluster and free atoms. This observation was made in the vicinity of the Xe 4d Cooper minimum, where the atomic angular distribution is known to