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Enzyme engineering of a haloacid dehalogenase-like phosphatase from Thermotoga neopolitana for optimization of substrate specificity

Haloacid dehalogenase (HAD)-like hydrolases represent a vast superfamily characterised by their ability to form covalent enzyme-substrate intermediates facilitating the cleavage of C-Cl, C-P, or CO-P bonds. Within this work one member of this superfamily, HAD from Thermotoga neopolitana (HADTn) exhibiting a phosphatase (P-O bond cleavage) like activity was investigated in the context of altering t

The role of odor receptor protein Or22a in Drosophila melanogaster oviposition and the response spectra of chimeric receptor protein Or22a-b

Doftreceptor Or22a och dess betydelse för äggläggning hos bananflugor Vanliga bananflugor har länge använts som testorganismer inom biologisk forskning, så som neurobiologi. Anledningen till detta är att de har ett nervsystem som är lättillgängligt men enklare uppbyggt än däggdjurs (Stocker, 1994,). Luktsinnet hos bananflugor sitter i antennerna och i de maxillära papillerna, som sitter vid flugaThe fruit fly Drosophila melanogaster has a well-studied olfactory system. A crucial part of the olfactory system is the olfactory sensory neurons (OSNs). One type of these OSNs, Ab3a, expresses the Odor receptors (ORs) Or22a and Or22b. Or22a is known to play an important role for behaviors like oviposition, but no strong ligand has been found for Or22b. Recent studies have shown that D. melanogas

Environmental Justice and Waste Management : location of landfills and socioeconomic conditions in São Paulo (SP, Brazil)

Environmentally safe disposal of urban waste is a global concern that recently gained a new dimension through the lens of Environmental Justice, with a growing literature showing correlation between the placement of waste facilities and disadvantaged groups in society. This thesis seeks to verify whether landfills in the city of São Paulo are located in regions where socially and economically vuln

Post-war CSR perspectives in Serbia

Purpose: With this study, an attempt was made to present an in-depth analysis of and study the interaction between PR agencies and their client’s CSR strategies in order to illuminate the contributions of public relations to these, and also to review current CSR activities applied in Serbia. Furthermore, the purpose was to foster the role of PR as an agent for change regarding CSR strategies. R

Numerical analysis of connection in wind turbine rotor blade

En växande världsbefolkning, politiska regleringar och en strävan att öka andelen energi från förnyelsebara källor har resulterat i att intresset för vindkraftsverk ökat. Winfoor AB utvecklar `Triblade’, en ny teknologi för vindkraftsverksblad som ämnar öka effekten samtidigt som bladen kan göras lättare, billigare och enklare att transportera än dagens vindkrafsverksblad.A globally growing population, an increased energy consumption and growing environmental awareness have made the interest for renewable energy sources, such as wind power, higher. As a result of the increased demand the need for new and improved technologies have increased. The energy production of a wind turbine is highly affected by the rotor blade length, which is limited by the massive dead lo

Rågsved Centre: Resilient Revitalisation in a Multi-Ethnic Environment

This report provides an analysis and evaluation of the current spatial, environmental and financial resilience of Rågsved Centre in Stockholm. Furthermore, local future risks on resilience are also discussed and the total analysis is synthesised in a new resilient spatial interpretation of the centre. Methods of analysis include a narrative format that comprise of twelve semi-structured interviews

Effect of pulsed electric field treatment in combination with hot-air drying on glandular trichomes of basil

Pulsed electric field (PEF) treatment is a non-thermal process which has a wide range of beneficial effects in food processing. The application of pulsed electric field treatment relevant for this work is as pre-treatment on basil leaves before the drying process. It has been proven that PEF treatment can significantly reduce the drying time due to the electroporation of the guard cells of stomata

Hur lämpliga är de geofysiska metoderna resistivitet och IP för kartläggning av PFOS?

PFOS (Perfluoroktansulfonat)-föroreningar har hittats på många ställen i Sverige och i resten av världen, därför finns det ett stort intresse och behov av att kunna kartlägga föroreningarna med fler metoder än vad som finns till-gängligt i dagsläget. Det här examensarbetet har som syfte att undersöka resistivitets- och IP (induced polarization)-mätningars lämplighet att kartlägga PFOS vid en brand

Testing aerosol vertical profiling methods with portable instruments on unmanned and manned aircraft

This thesis employed several portable sensors on board an unmanned aerial vehicle (UAV) and manned aircraft in order to test new methods of measuring vertical profiles of aerosol particles and contribute to knowledge of aerosol distributions in the atmosphere. More data in this area is required to improve atmospheric modelling and future climate scenarios. The handheld nanoparticle detector Partec

Characterization of NiMo catalysts with noble metal promoters

Klimatförändringar är en av vår tids stora utmaningar. För att kunna möta dessa finns det ett brådskande behov av att minska vart stora beroende av fossila bränslen. En lovande råvara för produktion av förnyelsebara och koldioxidneutrala bränslen är bioolja. Bioolja kan användas som ett substitut för råolja i raffinaderier och produceras från biomassa, vilket innebär att den kan agera som ett förnTo be able to face the challenges brought on by climate change there is an urgent need to reduce the heavy dependence on fossil fuels. Bio-oil is one such renewable and carbon-neutral replacement that has shown promising potential. A key step in making bio-oil commercially feasible is improvements of the upgrading processes of the bio-oil. One such upgrading process is catalytic hydrodeoxygenation

Unlocking the Potential of Solar-Hydro Hybrid Solutions: Feasibility and Economic Analysis

Hybrid solutions that combine multiple renewable energy sources, such as solar power and hydro power, are gaining popularity as a means of meeting the growing energy demands while minimising environmental impacts, but also as an opportunity of connecting solar power plants to the grid in areas where this might not be possible unless large investments are made. This master thesis aims to evaluate t

Exploring the Associations Between Socio-Digital Participation and Adolescent Well-Being

Det har uttryckts oro över ungdomarnas ständiga uppkoppling via sociodigitala teknologier och hur detta kan relateras till en upplevd försämring av unga människors välbefinnande. Få studier har fokuserat på totala användningen av både sociala medier och dataspel; eller har utforskat potentiella positiva samband mellan sociodigitalt deltagande (SDP) och ungdomarnas välbefinnande. Syfte: Den aktuellConcern has been voiced about adolescents’ instant connectivity via socio-digital technologies and how that relates to a perceived worsening of young people’s well-being. Few studies have focused on cumulative use of both social media and gaming; and/or exploring potential positive associations of socio-digital participation (SDP) and adolescent well-being. Objectives: The current study aimed to

Gömda rum till ytan

Vid första anblick ger Villacañas intrycket av att vara en anspråkslös by i centrala Spanien. Den besitter inte de karakteristiska egenskaperna som så många andra byar i Spanien har; det tydliga historiska arvet, gamla byggnader, små krokiga gator och gränder. I Villacañas är det första man möts av, till stor del, övergivna dörrfabriker och industrikomplex. Byns arkitektur är beige, rödbrun och daAt first sight, Villacañas seems like a dull and unassuming village in the centre of Spain. In contrary to so many other Spanish villages, Villacañas doesn’t have a strong and visible historical heritage with narrow streets and old buildings. The first thing that meets your eye, when entering the village, is an avenue of abandoned door factories and industrial complexes. The architecture of Villac

Sundvik Kollektivhus

Under de senaste fem åren har olika ekonomiska faktorer som påverkar bostadssituationen i Sverige ökat avsevärt, med skyhöga elpriser, bostadräntor och en hög inflation bl.a. Läget har gjort det omöjligt för främst unga, äldre eller människor i utanförskap att införskaffa en egen bostad. Samtidigt är landet ett av de länder som har flest ensamboenden i sina städer, vilket är en stor orsak till psyOver the past five years, various economic factors affecting the housing situation in Sweden have significantly increased, including skyrocketing electricity prices, housing interest rates, and high inflation, among others. This situation has made it impossible for primarily young people, the elderly, or individuals facing social exclusion to acquire their own homes. At the same time, the country

Impact on the Technical Performance of a Utility-Scale PV Plant when Using Different Sizes and Numbers of Transformers

Solcellsparker blir både allt vanligare och allt större. I denna studie undersöks hur storleken och antalet transformatorer i en solcellspark påverkar dennas förmåga att leverera el. De rådande klimatförändringarna är en av de största utmaningar mänskligheten ställts inför. Fossil elproduktion är en stor bidragande faktor till utsläpp av växthusgaser globalt. Solceller ser ut att spela en avgöranThe main focus of this thesis has been to examine and compare how the size and number of internal power transformers affects the technical performance (robustness, power quality and efficiency) of a utility-scale PV plant. The technical performance was examined in a simulation environment (MATLAB Simulink), where a PV plant with a fixed number of PV arrays and converters, corresponding to a rated

Evaluating the Benefits of Floating Container Terminals in Maritime Shipping: A Sustainability Perspective Using Facility Location Models

Study purpose: This study explores how Floating Container Terminals (FCTs) located in the North and Baltic Seas can support the maritime shipping industry's growth while promoting sustainability. As freight transportation volumes rise and sustainability regulations tighten, ports face increasing demands to accommodate Ultra-Large Container Vessels (ULCVs) in an environmentally responsible mann

Simulation of an HPC-process in Aspen HYSYS

Klimatförändringar är antagligen en av de största utmaningarna i modern tid, där förhöjda halter av växthusgaser i atmosfären har förödande konsekvenser på jordens livsuppehållande system. Koldioxid är ansedd som den mest utsläppta växthusgasen, vilket har lett till ett ökat intresse för koldioxidinfångning. Ett sätt för att minska de förhöjda halterna av koldioxid i atmosfären är genom koldioxidiWith climate change being one of the greatest challenges of our time, interest in carbon dioxide removal and mitigation has increased, as a way of minimizing emissions. As a result, carbon capture has become a key focus in the industry. One of the most common chemical absorption techniques is carbon capture using hot potassium carbonate (HPC). This process operates at an elevated absorber pressure

Learnings for Future

In the present time, the rate of environmental degradation requires us to use all available means to avoid further damage and restore the environment to the best of our possibilities. Education is an important channel to promote environmentalism and sustainability, and to promote pro-environmental individual decisions, especially regarding consumption and purchasing patterns. The case study analys

Synthesis of sialic acid 4-C derivatives through alkyne nucleophilic addition with the ambition to develop efficient inhibitors of bacterial transport proteins

Antimicrobial resistance is according to the World Health Organization (WHO) one of the most rapidly growing threats to world health. According to a recently published WHOreport, drug resistant microbials could be responsible for up to 10 million annual deaths by the year 2050 unless drastic measures are taken such as decreasing misuse and developing new antibiotics. This work explores the possibi