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Objective: To develop an enzyme linked immunosorbent assay (ELISA) to quantify the levels of specific aggrecan fragments generated by aggrecanase-mediated cleavage at the (373)Glu-(374)Ala bond within the aggrecan interglobular domain. Methods: The ELISA employs a commercially available monoclonal antibody to capture aggrecan fragments containing keratan sulfate (KS). Aggrecan fragments generated

We present a new method for quantification of the coupling efficiency between amphiphilic oligonucleotides and suspended small unilamellar lipid vesicles (SUVs). The method employs a supported (phospho) lipid bilayer (SLB)-modified sensor template, which upon exposure to a mixture of SUVs and amphiphilic DNA reacts neither with free SUVs nor with DNA-modified SUVs, but with free DNA only. Using ca

Previous cytogenetic and comparative genomic hybridization (CGH) analyses have shown that the gain of chromosome arm 12p is frequent in pancreatic carcinomas. We investigated 15 pancreatic carcinoma cell lines using CGH, fluorescence in situ hybridization (FISH), and semiquantitative polymerase chain reaction (PCR) to characterize 12p amplifications in detail. The CGH analysis revealed gains of 12

The binding of factor IX to cultured bovine endothelial cells was characterized using isolated domains of bovine factor IX. An NH2-terminal fragment that consists of the gamma-carboxyglutamic acid (Gla) region linked to the two epidermal growth factor (EGF)-like domains bound to the endothelial cells with the same affinity as intact factor IX, indicating that the serine protease part of factor IX

1. Using data from a 9-year study of the great reed warbler Acrocephalus arundinaceus L. carried out in Sweden, I tested the main assumption of the polygyny threshold model (that there is a cost of polygyny) and the main prediction (that this cost is compensated for). The main question was whether a female that chose a mated male (secondary females) experienced the same fitness as a female that at

[1] Mires are key landscape elements at high latitudes and have certainly accumulated carbon during the Holocene, but their current carbon balance at the present time is very unclear. The major carbon flux is the land-atmosphere CO2 exchange and full-year data are still limited. Here we present data from 3 a (2001-2003) of continuous Eddy Covariance measurements at Degero Stormyr (64 degrees 11'N,

The measured net ecosystem exchange (NEE) of CO2 between the ecosystem and the atmosphere reflects the balance between gross CO2 assimilation [gross primary production (GPP)] and ecosystem respiration (R-eco). For understanding the mechanistic responses of ecosystem processes to environmental change it is important to separate these two flux components. Two approaches are conventionally used: (1)

The preferences of some woodland understorey species for ammonium and nitrate were investigated by measuring the potential nitrification (conversion of ammonium to nitrate) in the rhizosphere compared with the bulk soil. Less acid-tolerant species, which usually prefer nitrate or a mixture of ammonium and nitrate in hydroponic culture, should have a higher potential nitrification in the rhizospher

Western style diets and lifestyles are associated with increasing rates of obesity, diabetes and insulin resistance. Higher circulating insulin levels may modulate cell proliferation and apoptosis either directly or indirectly by increasing the bioactivity of IGF-I and decreasing the bioactivity of some of its binding proteins. The objective of this study was to determine the association of increa

The structural basis for the biological specificity of human cystatin C has been investigated. Cystatin C and other inhibitors belonging to family 2 of the cystatin superfamily interact reversibly with target peptidases, seemingly by independent affinity contributions from a wedge-shaped binding region built from two loop-forming inhibitor segments and a binding region corresponding to the N-termi

Endowed with diverse vegetation covers, the Middle Atlas Mountains receive substantial snowfall in winter, which contributes to plant hydration during the growing season. Using remotely-sensed imagery, this study aims to assess the trends in snow cover variations between 2000 and 2015, analyse the local influence of snowpack persistence on vegetation growth and evaluate the significance of this ef

Antibodies have for long time dominated the field of affinity proteins in example imaging and therapeutics, due to their high specificity and affinity. However, they have some major drawbacks such as high molecular weight, complex structure leading to instability and high production cost. Therefore, other types of affinity proteins have been engineered, for example the Affibody molecule which is a

 Skeptisk filosof – ateist eller agnostiker?  Religiösa trosföreställningar behövs inte — vare sig för vetenskapens eller moralens skull  Varför religionsfilosofi? Befria mänskligheten från vidskepelse och fördomar Förstå religiösa trosföreställ- ningars ursprung och funktion Religiositet är inte en primär eller ursprunglig mental inställning – ingår inte i människans naturliga konstitution som

https://www.fil.lu.se/media/utbildning/dokument/kurser/FPRA11/20141/Rel2.pdf - 2026-05-31

Today, "the digital library" is the common way of describing a managed collection of information, with associated services, stored in digital formats and accessible over a network. Digital libraries have mainly been developed at research libraries, but during the last few years public libraries have started to offer similar services. The aim of this Master's thesis is to study how t

Rel2.pptx 14-05-28 1  Skeptisk filosof – ateist eller agnostiker?  Religiösa trosföreställningar behövs inte — vare sig för vetenskapens eller moralens skull  Varför religionsfilosofi? Befria mänskligheten från vidskepelse och fördomar Förstå religiösa trosföreställ- ningars ursprung och funktion Religiositet är inte en primär eller ursprunglig mental inställning – ingår inte i människans natur

https://www.fil.lu.se/media/utbildning/dokument/kurser/FPRA11/20141/Rel2_.pdf - 2026-05-31