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Surface plasmon resonance for real-time study of lectin-carbohydrate interactions for the differentiation and identification of glycoproteins.

A study of specific interactions between lectins and glycoproteins has been carried out using surface plasmon resonance (SPR) in a flow-injection mode. Lectins were covalently immobilised on the surfaces of the microfluidic sensor chip via amine coupling and serum glycoproteins were injected into the flow channels. Specific lectin-glycoprotein interactions caused the shift of refractive index prop

Timing Diagnostics and Coherent Harmonics from a test-FEL

Popular Abstract in Swedish I denna avhandling ges en introduktion till fyra artiklar där resul- tat från och delar av en frielektron-laser (FEL) beskrivs. Denna typ av laser skiljer sig från de konventionella lasrar de flesta av oss har träffat på, t.ex. i form av en laserpekare. Den första märkbara skill- naden är att frielektron-lasrar är stora, ofta flera hundra meter långa. De kräver en accelThe test free-electron laser (test-FEL) at MAX-lab in Lund demonstrated for the first time circularly polarized coherent femtosecond pulses at 66 nm wavelength. A 375 MeV electron bunch was seeded by a femtosecond laser at 263 nm and coherent harmonics were extracted in an APPLE-II type elliptical undulator. A thermionic gun with a barium oxide cathode was adapted for photocathode operation, and t

The aqueous phase behavior of polyion-surfactant ion complex salts mixed with nonionic surfactants.

The aim of this work was to study intermolecular interactions in systems containing charged polyion (polyacrylate, PA(-)), charged surfactant (C(16)TA(+)) and nonionic surfactant (C(12)E(5) or C(12)E(8)). To achieve this we have created four different phase diagrams using two different so-called complex salts, C(16)TAPA(25) and C(16)TAPA(6000), both consisting of positively charged surfactant (C(1

Hydrogen Production for Refuelling Applications

Since 2007, Intelligent Energy (IE) has been involved in developing technologies and services in the hydrogen generation space with E.ON Gas Sverige AB and Swedish Gas Centre. The aim of this work is to support the development of a high-profile demonstration of hydrogen generation technologies in a Swedish context. The overall objective of the demonstration is to deploy a reforming based hydrogen

Selective determination of acidic pharmaceuticals in wastewater using molecularly imprinted solid-phase extraction.

The determination of acidic pharmaceuticals, such as non-steroidal anti-inflammatory drugs NSAIDs and clofibric acid (metabolite of clofibrate), at low ngL(-1) levels in wastewater requires highly selective and sensitive analytical procedures. The removal of matrix components during sample preparation results in significant benefits towards reducing the matrix effects during LC-MS analysis. Theref

Multivariate analysis of chromosomal imbalances in breast cancer delineates cytogenetic pathways and reveals complex relationships among imbalances.

More than 550 breast adenocarcinomas with clonal chromosomal abnormalities have been reported. Although the aberration pattern is clearly nonrandom, no specific primary or secondary karyotypic abnormality has been identified, and furthermore the chronological order in which the aberrations appear during disease progression is not well known. The high degree of karyotypic complexity in epithelial t

Small Distal Muscles and Balance Predict Survival in End-Stage Renal Disease.

Background/Aims: Survival for patients on renal replacement therapy (RRT) has been shown to correlate to the level of physical activity and exercise capacity. We examined whether composite measures of functional status at the start of RRT predict survival. Methods: In this retrospective study, the same physiotherapist, using a standardized battery of tests for functional status, tested 134 patient

Light and circadian regulation of clock components aids flexible responses to environmental signals

The circadian clock measures time across a 24h period, increasing fitness by phasing biological processes to the most appropriate time of day. The interlocking feedback loop mechanism of the clock is conserved across species; however, the number of loops varies. Mathematical and computational analyses have suggested that loop complexity affects the overall flexibility of the oscillator, including

Biasing moral decisions by exploiting the dynamics of eye gaze

Eye gaze is a window onto cognitive processing in tasks such as spatial memory, linguistic processing, and decision making. We present evidence that information derived from eye gaze can be used to change the course of individuals’ decisions, even when they are reasoning about high-level, moral issues. Previous studies have shown that when an experimenter actively controls what an individual sees

Spectral sensitivity in Onychophora (velvet worms) revealed by electroretinograms, phototactic behaviour and opsin gene expression.

Onychophorans typically possess a pair of simple eyes, inherited from the last common ancestor of Panarthropoda (Onychophora + Tardigrada + Arthropoda). These visual organs are thought to be homologous to the arthropod median ocelli, whereas the compound eyes most likely evolved in the arthropod lineage. To gain insights into the ancestral function and evolution of the visual system in panarthropo

Orally Administered Manganese With and Without Ascorbic Acid as a Liver-Specific Contrast Agent and Bowel Marker for Magnetic Resonance Imaging: Phase I Clinical Trial Assessing Efficacy and Safety.

OBJECTIVES:: The objectives of this clinical trial of orally administered manganese in magnetic resonance imaging (MRI) of the liver were to assess signal enhancements in the liver with and without the addition of an uptake promoter, ascorbic acid, and to evaluate acute safety. MATERIALS AND METHODS:: A total of 18 healthy adult males were enrolled in the present trial. Contrast medium: MnCl2, dos

Nonparametric methods for microarray data based on exchangeability and borrowed power

This article proposes nonparametric inference procedures for analyzing microarray gene expression data that are reliable, robust, and simple to implement. They are conceptually transparent and require no special-purpose software. The analysis begins by normalizing gene expression data in a unique way. The resulting adjusted observations consist of gene-treatment interaction terms ( representing di

Development of a PCR-compatible enrichment medium for Yersinia enterocolitica: amplification precision and dynamic detection range during cultivation

A Yersinia PCR-Compatible Enrichment (YPCE) medium was developed, which removes the necessity for sample pretreatment before PCR-based detection of Yersinia enterocolitica. The medium was designed through a sequence of independent screening and factorial design experiments to study the PCR inhibition and growth characteristics of medium components. The compatibility of the YPCE medium was evaluate

Multilamellar Vesicle Formation from a Planar Lamellar Phase under Shear Flow

The formation of multilamellar vesicles (MLVs) from the lamellar phase of nonionic surfactant system C12E5/D2O under shear flow is studied by time-resolved small angle neutron and light scattering during shear flow. A novel small angle neutron scattering sample environment enables the tracking of the lamellae alignment in the velocity-velocity gradient (1-2) plane during MLV formation, which was t

Nonlinear compartmental model of F-18-choline

Introduction: This work develops a compartmental model of F-18-choline in order to evaluate its biokinetics and so to describe the temporal variation of the radiopharmaceuticals' uptake in and clearance from organs and tissues. Methods: Ten patients were considered in this study. A commercially available tool for compartmental analysis (SAAM II) was used to model the values of activity concentrati

Steady-state analysis of activated sludge processes with a settler model including sludge compression

A reduced model of a completely stirred-tank bioreactor coupled to a settling tank with recycle is analyzed in its steady states. In the reactor, the concentrations of one dominant particulate biomass and one soluble substrate component are modelled. While the biomass decay rate is assumed to be constant, growth kinetics can depend on both substrate and biomass concentrations, and optionally model

Quality and readability of English-language internet information for adults with hearing impairment and their significant others

Objective: This study evaluated the quality and readability of English-language internet information for adults with hearing impairment and their significant others. Design: Two keyword pairs (hearing loss and hearing aids) were entered into five country-specific versions of the most commonly used internet search engine in May 2011. Sample: For each of the 10 searches, the first 10 relevant websit

Assessment of oxidative burst in avian whole blood samples: Validation and application of a chemiluminescence method based on Pholasin

Immunoecology deals with the questions about how immune defences have evolved and are being used and optimized in different environments, ecological settings and lineages. In such research, often only single time point measures and small sample volumes are available, which limits the applicability of traditional immunological methods. Methodological progress in the field thus largely depends on th