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Peptide mass fingerprinting (PMF) has over the years become one of the most commonly used tools for high-throughput analysis and identification of proteins. This method is applicable when relatively simple samples have to be analysed and it is commonly used for analysing proteins previously separated by 2-DE. The most common type of instrument used for this approach is the MALDI-TOF that has prove
We studied the energy and protein balance of a Thrush Nightingale Luscinia luscinia, a small long-distance migrant, during repeated 12-hr long Eights in a wind tunnel and during subsequent two-day fueling periods. From the energy budgets we estimated the power requirements for migratory flight in this 26 g bird at 1.91 Watts. This is low compared to flight cost estimates in birds of similar mass a
Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the
The Siberian Jay (Perisoreus infaustus) lives in resident, territorial family groups outside the breeding season, but does not breed cooperatively. Thereby it offers an opportunity to study the evolution of territorial group living, without confounding effects of reproductive cooperation. During a long-term study in Finland 1974-2000, we observed Siberian Jay group composition in autumn. Using mic
Bile-tolerant Helicobacter species such as Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (CLD) in humans. Antibody responses to cell surface proteins of H. pullorum, H. bilis, and H. hepaticus in serum samples from patients with CLD, a randomized population gro
Neutrophil migration across infected mucosal surfaces is chemokine dependent, but the role of chemokine receptors has not been investigated. In this study, chemokine receptors were shown to be expressed by epithelial cells lining the urinary tract, and to play an essential role for neutrophil migration across the mucosal barrier. Uroepithelial CXCR1 and CXCR2 expression was detected in human urina
Hydration of hen egg white lysozyme was studied by using the method of sorption calorimetry at 25, 40, and 50degreesC. Desorption calorimetric measurements were performed at 25 and 40degreesC. The activity of water and partial molar enthalpy of mixing of water were determined as functions of water content. Hydration of lysozyme occurs in four steps: slow penetration of water into the protein-prote
The internal structure of composite gels made of responsive microgel particles inserted into a bulk hydrogel (N-isopropylacrylamide microgel particles in a cross-linked dimethylacrylamide matrix) has been investigated from the diffusion behavior of poly(ethylene glycol) (PEG) probes through the network, in the absence of specific interactions between the diffusing molecules and the system. The eff
The aqueous phase behavior of a series of complex salts, containing cationic surfactants with polymeric counterions, has been investigated by visual inspection and small-angle X-ray scattering (SAXS). The salts were alkyltrimethylammonium polyacrylates, C(x)TAPA(y), based on all combinations of five surfactant chain lengths (C-6, C-8, C-10, C-12, and C-16) and two lengths of the polyacrylate chain
Pavements are constructed using several layers of materials, and their durability depends mainly on the stiffness modulus and thickness of these strata. Surface wave testing is an effective tool to measure the stiffness and thickness of pavement layers. However, measurements are still based on spot testing with fixed receivers and source. The typical large size of pavements and the cost of closing
Social media platforms open up new methods of candidate messaging, voter contact, and data acquisition. Currently, affordances is a popular concept used to study the relationship between political campaigns and how they use social media platforms. Scholars argue that since platforms have different designs, they offer different affordances that affect how campaigns use social media. This chapter ev
Marginal zone B cells (MZB cells) are innate like B cells that are predominantly located alongside memory B cells in the microanatomical region of the spleen of humans and mice termed the marginal zone. They circulate in humans but not mice, and in humans can be identified in blood and other lymphoid tissues, including the gut-associated lymphoid tissue, where they, as in spleen, form a zone surro
OBJECTIVE: To study the association between sperm deoxyribonucleic acid fragmentation index (DFI) and the odds of preeclampsia and other adverse perinatal outcomes after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment.DESIGN: A prospective cohort study including infertile couples undergoing conventional IVF or ICSI treatment and their children. Data regarding pre
The aim of this thesis is to investigate whether tort liability claims against public authorities for breaches of the ECHR can be defined as a constitutional right in Swedish law. The theoretical definition of what constitutes a constitutional right is constructed through elements of Robert Alexy’s A Theory of Constitutional Rights. The theory is applied on the current applicable law regarding the
