Sökresultat

Processing and presentation of insulin. : II. Evidence for intracellular, plasma membrane-associated and extracellular degradation of human insulin by antigen-presenting B cells

To study the biochemistry of processing of a soluble protein Ag by an APC, we investigated how 125 I-labeled human insulin (HI) is processed in situ by TA3 mouse hybridoma B cells. Fractionation of TA3 cells into their extracellular, plasma membrane-associated and intracellular compartments coupled with the use of HPLC enabled us to analyze several peptides derived from each compartment. One HI

Purification and characterization of radiolabelled biosynthetic human insulin from Escherichia coli kinetics of processing by antigen presenting cells

An Escherichia coli strain transfected with a plasmid containing four linked human proinsulin genes was grown in the presence of 35S and 3H labelled amino acids to gain access to human insulin that was radiolabelled at 19 evenly distributed sites throughout the amino acid sequence. The multi-proinsulin precursor was cleaved at methionine residues with cyanogen bromide, then the individual proinsul

Generation of an induced pluripotent stem cell line (CSC-32) from a patient with Parkinson's disease carrying a heterozygous variation p.A53T in the SNCA gene

Here, we describe the generation of an induced pluripotent stem cell (iPSC) line, from a male patient diagnosed with Parkinson's disease (PD). The patient carries a heterozygous variation p.A53T in the SNCA gene. Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated iPSC line (CSC-32) preserved the mutati

Master protocols in clinical trials : a universal Swiss Army knife?

Master protocols combine several sub-trials, each with their own research objectives, which is usually presented as one single clinical trial application. Master protocols have become increasingly popular in oncology and haematology, as either basket, umbrella, or platform trials. Although master protocols are intended to accelerate drug development and to reduce futility, their use poses challeng

Furstar, nya skepp och början på det moderna

Polymorphism of normal factor IX detected by mouse monoclonal antibodies

Assessment and recognition of learning and training

Future development of biogas systems in Sweden – incentives and barriers.

Summing up five years of research on children’s experience of and exposure to online advertising

New insights into mobility and affinity of engineered family 4 CBMs in bioinspired lignocellulosic assemblies

Adaptation and anticipation: Learning from experience

Expanding Bioenergy in Europe

Despite apparently favourable conditions for investment in the bioenergy sector provided by sustained high oil prices and a multitude of policy support measures throughout the EU that are ostensibly intended to create improved economic conditions, many industrialists and investors are still apparently wary of engagement. National policy interventions intended to support and to expand the bioenergy

Is Verbal Retrospective of Eye-Movements a Better Method for Evaluating Automobile Interior Design?

Relative importance of secondary settling tank models in WWTP simulations - A global sensitivity analysis using BSM2

Nationality vs. Domicile as Connecting Factor in European Private International Law in Family Matters

Necropolitics and the Gender of Colonial Violences

Leadership skills and the use of conceptual models

Modulation of the effect of Dextran Sulphate Sodium (DSS)-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium

The IgE repertoire in grass pollen allergy – specificity and diversity

Collecting data for quality improvement and research in Swedish health care, and the individual patient's right and ability to protect their privacy