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The scFv antibody towards the Burkholderia pseudomallei exotoxin was previously constructed by phage display and exhibited good specificity towards the exotoxin. We report here the optimization of the scFv expression in an E. coli expression system. Four different E. coli strains (ER2537, TG1, HB2151, and XL1-Blue) were examined for optimal expression of the scFv protein. Two types of carbon sourc

The worsening of psychical health issues among young people has widely been discussed over the last few years. Research shows for example that 9% of girls at the age of 16-24 reported anxiety problems in 1989 while 16 years later, in 2005, the same number was 30%. The purpose of this essay was to study and analyze psychiatrists', psychologists' and counselors' view on the resilience an

The optimal selection of characteristic bands and retrieval models for the hyperspectral retrieval of soil heavy metal concentrations poses a significant challenge. Additionally, satellite-based hyperspectral retrieval encounters several issues, including atmospheric effects, limitations in temporal and radiometric resolution, and data acquisition, among others. Given this, the retrieval performan

Discrepancies in views of the Self are suggested to be negatively related to well-being (Higgins, 1987). In the present study, we used a novel concept, Personality Estimation Discrepancy (PED), to test this classic idea. PED is defined as the computed difference between how one view oneself (Self-Perceived Personality) and a standard Big Five test (IPIP-NEO-30). In a pre-registered (osf.io) UK onl

The healthcare environment may seem frightening and overwhelming in times when life-threatening conditions affect a family member or close friend and individuals visit the patient in an ICU. A two-bed patient room was refurbished to enhance the well-being of patients and their families according to the principles of evidence-based design (EBD). No prior research has used the Person-centred Climate

The investigation is concentrated on two important quantities – the Strouhal number and the mean base suction coefficient, both measured at the mid-span position. Reynolds numbers from about 50 to 4 × 104 were investigated. Different aspect ratios, at low blockage ratios, were achieved by varying the distance between circular end plates (end plate diameter ratios between 10 and 30). It was not pos

The current study focused on the surface integrity issues associated with broaching of Inconel 718 and the structural stability of the broached specimen at high temperatures, mainly involving the microstructural changes and residual stress relaxation. The broaching operation was performed using similar cutting conditions as that used in turbo machinery industries for machining fir-tree root fixing

Softwood bark contains a large amounts of extractives—i.e., soluble lipophilic (such as resin acids) and hydrophilic components (phenolic compounds, stilbenes). The effects of the partial removal of water-soluble extractives before acid-catalyzed steam pretreatment on enzymatic digestibility were assessed for two softwood barks—Norway spruce and Scots pine. A simple hot water extraction step remov

Physiotherapists are facing complex health challenges in the treatment of persons suffering from long-lasting musculoskeletal disorders and mental health problems. Basic Body Awareness Therapy (BBAT) is a physiotherapy approach within the movement awareness domain developed to bridge physical, mental, and relational health challenges. The purpose of this study was to reach a consensus on core phen

Popular Abstract in Swedish Denna avhandling föreslår förbättringar av vissa verktyg som svenska myndigheter använder för samhällssäkerhet och krishantering; krishanteringsutvärderingar, krishanteringsövningar samt organisatoriska riskbedömningar. Avhandlingen undersöker även hur visualiseringar av säkerhetskulturdata kan stödja utveckling av säkerhetskultur. Forskningen motiverades av praktiska bThis thesis suggests improvements of selected parts of the Swedish authorities’ toolkits for societal safety and crisis management; crisis response evaluations, crisis management exercises and organizational risk assessments. The thesis also explores how visualizations of safety culture data can be used to support safety culture development. The research was motivated by practical needs and delive

Cationic vinylpyridine based molecularly imprinted polymer monoliths were, for the first time, applied to enantiomer separation using capillary electrochromatography. In order to map the synthesis conditions under which superporous monoliths are obtained, capillary columns were prepared by an in situ polymerization technique using varying monomer and porogen compositions. Both electrolyte pH and p

As new therapeutic areas in which genetic material is employed, i.e. gene therapy and DNA vaccination, are expanding, a substantial increase in the demand for gene vectors, e.g. plasmid DNA, is foreseen. Chromatography, being a high-resolution method, is considered essential for the purification of gene vectors when high purity is required. Plasmids are large molecules, which cannot penetrate the

Prenatal testosterone surge is considered crucial for physiological masculinization of male progeny. Disorders in sex steroid hormone balance during the fetal development may interfere with male reproductive health later in life. In this study, we have investigated in utero and in vitro effects of flutamide (FLU) and diethylstilbestrol (DES) on fetal rat testicular steroidogenesis. In utero exposu

Questions: How does the number of species in a community affect the level of genetic diversity of its constituent species? What is the relation between competitive interactions and the spatial distribution of genetic variation? When spatially structured, how is the genetic differentiation among subpopulations affected by competition and size of the community? Model features and key assumptions: A

Moisturizing creams have beneficial effects in the treatment of dry, scaly skin, but they may induce adverse skin reactions. In a randomized double-blind study, 197 patients with atopic dermatitis were treated with one of the following: a new moisturizing cream with 20% glycerin, its cream base without glycerin as placebo, or a cream with 4% urea and 4% sodium chloride. The patients were asked to

Clinical techniques for early detection of acute hypotension during conventional hemodialysis treatment are lacking, even though intradialytic hypotension is the most common acute complication. In this article, intradialytic hypotension is identified by means of signal analysis of data recorded at two clinics. The database consists of 30 treatments with concurrently acquired signals: the 12-lead e

The radiation exposure of fingers, thyroid and eyes of workers handling radiopharmaceuticals during various nuclear medicine procedures was measured using thermoluminescent dosemeters. Dosemeters were placed on the finger tips of 19 workers on several different occasions for various procedures. Additionally, the routinely determined whole-body doses to various groups of workers were analysed. The

Total antioxidant capacity (TAC), total phenolic compounds (TPH), total flavonoids (TF) and the amounts of individual phenolic compounds were assessed in Bolivian potato cultivars (various Solanum species) before, during and after the traditional freezing and sun-drying of potatoes known as chunos. The TAC of chunos ranged from 0.4 to 2.7 mu mol Trolox equivalents/g dry matter using 2,2'-azino-bis