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We have purified wild type, three structural stability mutants and four charge mutants of bacteriophage T4 lysozyme from E. coli strains harboring desired expression vectors. Structural stability mutants were produced by substitution of the isoleucine at amino acid position three, yielding a set of proteins with stabilities ranging from 1.2 kcal/mol greater, to 2.8 kcal/mol less, than that of the

We present evidence of a submerged early Holocene landscape off the Blekinge coastline in the Baltic Sea, dating to the Yoldia Sea and Initial Littorina Sea Stages when the water level was lower than at present. 14C dated wood remains obtained by surveillance diving and new archaeological findings in combination with bathymetric analyses and interpolations between other sites across the Baltic Sea

In the formulation of an active enzyme enclosed in a matrix for controlled delivery, it is a challenge to achieve a high protein load and to ensure high activity of the protein. For the first time to our knowledge, we report the use of a highly swollen lipid sponge (L3) phase for encapsulation of the large active enzyme, β-galactosidase (β-gal, 238 kDa). This enzyme has large relevance for applica

The shear plate dowel joint (SPDJ) is a novel connection design for heavy timber structures. The connection uses a single large diameter dowel and glued-on steel plates to transfer loads between members. Consequently, the design of the dowel is of great influence for the global performance of the connection. This paper presents an experimental and numerical investigation of the influence of the ge

We show that the permanent of an n × n matrix over any finite ring of r ≤ n elements can be computed with a deterministic 2n−Ω(nr ) time algorithm. This improves on a Las Vegas algorithm running in expected 2n−Ω(n/(r log r)) time, implicit in [Björklund, Husfeldt, and Lyckberg, IPL 2017]. For the permanent over the integers of a 0/1-matrix with exactly d ones per row and column, we provide a deter

Context: The global software industry and the software engineering (SE) academia are two large communities. However, unfortunately, the level of joint industry-academia collaborations in SE is still relatively very low, compared to the amount of activity in each of the two communities. It seems that the two ’camps’ show only limited interest/motivation to collaborate with one other. Many researche

Background: Physical activity reduces the risk of acquired cardiovascular disease, which is of great importance in patients with congenital heart disease (CHD). There are diverging data whether physical activity level (PAL) differs between patients with CHD and controls. Furthermore, it is unknown if PAL can be reliably assessed in patients with CHD using self-reported instruments. Methods: Sevent

The paper focuses on skills and competences used in audio description (AD) of a Swedish popular scientific journal. The journal, containing complex images and visualizations, is made accessible for visually impaired audiences by producing an audio version. The task is to create a verbal description to enhance understanding and enjoyment. The interpretative process of meaning-making is uncovered by

To date, the best daylighting assessment technique is provided by climate-based simulation tools, which require remarkable efforts to create and calibrate realistic models. The data-driven approaches represent an interesting opportunity to support the physics-based modelling. This work proposes a novel method aimed at the optimization of energy use and luminous environment for a set of lighting co

Landscape heterogeneity is an important driver of biodiversity in agroecosystems. However, the functional heterogeneity of agricultural landscapes, taking into account the different resources that habitat patches can provide to species, has rarely been studied. In this study, we explored the effect of landscape-scale nest availability provided by permanent grasslands on wild bee communities. Wild

Colonization and migration have a crucial effect on patterns of biodiversity, with disease predicted to play an important role in these processes. However, evidence of the effect of pathogens on broad patterns of colonization and migration is limited. Here, using phylogenetic analyses of 1,311 species of Afro-Palaearctic songbirds, we show that colonization events from regions of high (sub-Saharan

Peptide self-assembly is a spontaneous process, ubiquitous in nature for the creation of highly ordered and complex biological structures, making it a sought after technique in the development of novel biocompatible materials. The process is also strongly associated with various neurodegenerative diseases in the formation of amyloid plaques in diseases such as Alzheimer’s and Parkinson’s, where fu

Autoantibodies against 21-hydroxylase (P450c21) are common in idiopathic autoimmune Addison’s disease. In the present work, we have developed a sensitive radiobinding assay using in vitro translated recombinant human 35S-P450c21. Levels of P450c21 antibodies (P450c21-Ab) were expressed as a relative index (P450c21 index) using a P450c21-Ab positive Addisonian serum and two antibody-negative health

Hypothesis: The self-assembly of long tail sugar-based surfactants into worm-like micelles has recently been demonstrated, and the rheological properties of such systems have been shown to be tuneable through subtle modifications of the molecular characteristics of the surfactant monomer. In particular, the anomeric configuration of the hexadecylmaltoside headgroup was shown to induce profound cha

The prediction of acoustic propagation from a monofrequency coherent line source in a cutting with impedance boundary conditions over a noise-screen onto surrounding flat grassland is presented. It is well known that over flat ground the spectra for single-noise screens have significant marked differences for propagation over absorbing ground where the screen obstructs surface wave attenuation ove

Climate change and human activities influence the development of ecosystems, with human demand of ecosystem services altering both land use and land cover. Fossil pollen records provide time series of vegetation characteristics, and the aim of this study was to create spatially continuous reconstructions of land cover through the Holocene in southern Sweden. The Landscape Reconstruction Algorithm