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Lymphocytes from human peripheral blood were separated by Countercurrent distribution (CCD) in a charged aqueous two-phase system composed of Dextran T 500 and polyethylene glycol 6000. Maximal responses to the T-cell mitogens phytohemagglutinin and concanavalin A were detected in a part of the distribution corresponding to the area where the highest percentage of E rosetting cells were seen. Anti
Protein ADP-ribosylation is a reversible post-translational modification that regulates important cellular functions. The identification of modified proteins has proven challenging and has mainly been achieved via enrichment methodologies. Random mutagenesis was used here to develop an engineered Af1521 ADP-ribose binding macro domain protein with 1000-fold increased affinity towards ADP-ribose. T
We have characterized 2 distinct mechanisms through which infectious agents may promote platelet adhesion and thrombus formation in flowing blood, thus contributing to the progression of disease. In one case, the process initiates when the integrin alpha(IIb)beta(3) mediates platelet arrest onto immobilized bacterial constituents that have bound plasma fibrinogen. If blood contains antibodies agai
Mangrove ecosystems have the potential to provide a host of ecosystem services to the communities surrounding them. Dense, healthy, networks of mangroves are able to provide a buffer to coastlines against the increasingly frequent and intense storms, expected under the influence of climate change. Additionally, healthy, mangrove ecosystem provide coastal communities with other benefits such as mor
The planetary changes associated with the Anthropocene, including climate change and extinction of species, pose severe threats to civilization, humanity, and the natural world as we know it. They also pose special challenges to the human imagination. To meet these challenges, climate change communicators use narratives. Nonfiction books intended for a general audience employ two radically differe
EndoS from Streptococcus pyogenes efficiently hydrolyzes the functionally important and conserved N-linked glycan of IgG in human blood. Repeated i.v. administration of EndoS in rabbits completely hydrolyzes the glycans of the whole IgG pool, despite the generation of anti-EndoS antibodies. EndoS administration had no apparent effects on the health of the animals. EndoS hydrolysis of the IgG glyca
Group B Streptococcus (GBS) is a leading cause of invasive bacterial infections in human newborns. A key GBS virulence factor is its capsular polysaccharide (CPS), displaying terminal sialic acid (Sia) residues which block deposition and activation of complement on the bacterial surface. We recently demonstrated that GBS Sia can bind human CD33-related Sia-recognizing immunoglobulin (Ig) superfami
Decarbonizing the energy-intensive and natural resource-based industries is possible but may sub-stantially increase the cost of production. Whether such cost increases will reduce economic welfare depends on how the downstream industries respond to the higher cost for intermediate goods. In this paper, we explore how downstream industries in the EU15 responded to upstream carbon technology shocks
This work aims at studying photoionization dynamics from atoms and surfaces at attose time scales. The work was based on development and applications of a high repetition rate High-order Harmonic Generation (HHG) light source that utilizes Optical Parametric Amplification (OPA) laser technology. The laser system delivers few-cycle pulses in the near infrared (IR) at a repetition rate of 200 kHz. TThis work aims at studying photoionization dynamics from atoms and surfaces at attose time scales. The work was based on development and applications of a high repetition rate High-order Harmonic Generation (HHG) light source that utilizes Optical Parametric Amplification (OPA) laser technology. The laser system delivers few-cycle pulses in the near infrared (IR) at a repetition rate of 200 kHz. T
