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Targeted measurements of low abundance proteins in complex mixtures are in high demanded in many areas, not the least in clinical applications measuring biomarkers. We here present the novel platform AFFIRM (AFFInity sRM) that utilizes the power of antibody fragments (scFv) to efficiently enrich for target proteins from a complex background and the exquisite specificity of an SRM-MS based detectio
Background: Stress ulcer prophylaxis (SUP) may decrease the incidence of gastrointestinal bleeding in patients in the intensive care unit (ICU), but the risk of infection may be increased. In this study, we aimed to describe SUP practices in adult ICUs. We hypothesised that patient selection for SUP varies both within and between countries. MethodsAdult ICUs were invited to participate in the surv
Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by haploinsufficiency of genes encoding ribosomal proteins (RPs). Perturbed ribosome biogenesis in DBA has been shown to induce a p53-mediated ribosomal stress response. However, the mechanisms of p53 activation and its relevance for the erythroid defect remain elusive. Previous studies have indicated that activation of p53
Abstract Non-profit organisations are under increasing pressure to demonstrate their social impact. This paper examines the experience and behaviour of non-profit organisations in the UK in relation to a demand for social impact evaluations. External resource providers request organisations to present evidence on how resources are used and what organisations have achieved. While most organisations
Groups of spiritual practitioners (P) (n = 38), long-term residents in a center offering spiritual courses (R) (n = 8), and matched control groups from a general, population sample for P (CP) (n = 45) and for R (CR) (n = 14) were compared. P had high levels of education and tended to be in a committed relationship and belong to the Danish National Church, whereas R had a lower level of education a
The ability to design and implement silk feedstock formulations for tailored spinning has so far eluded the bioengineers. Recently, the high throughput screening technique of differential scanning fluorimetry (DSF) demonstrated the link between the instability transition temperature (Ti) and the processability of the silk feedstock. Using DSF we screened a large set of chemicals known to affect so
Lymphoplasmacytic lymphoma (LPL)/Waldenstrom macroglobulinemia (WM) is a B-cell disorder resulting from the accumulation, predominantly in the bone marrow, of clonally related lymphoplasmacytic cells. LPL/WM is a very rare disease, with an incidence rate of 3-4 cases per million people per year. Currently, the causes of LPL/WM are poorly understood; however, there are emerging data to support a ro
Objectives To determine the frequency and nature of erroneous transfer of medication information (medication errors) upon admission to hospital and to study the effect of medication reconciliation. Methods Included patients were 65years of age or older, were living in nursing homes or in their own home with care provided by the community nursing system and had been admitted to hospital. The patien
Spider silk has been studied extensively for its attractive mechanical properties and potential applications in medicine and industry. The production of spider silk, however, has been lagging behind for lack of suitable systems. Our approach focuses on solving the production of spider silk by designing, expressing, purifying and characterizing the silk from cylindriform glands. We show that the cy
AIMS: To determine whether the restrictive physiology seen in Tetralogy of Fallot (TOF) patients can be explained by fibrosis of the right ventricular (RV) outflow tract. The aetiology for restrictive RV physiology after TOF repair is not known. METHODS AND RESULTS: TOF patients (n = 31, 13 girls, 10.2 years ± 2.8) were included 9.2 ± 2.9 years after total correction and examined with cardiac magn
Targeted proteomics allows researchers to study proteins of interest without being drowned in data from other, less interesting proteins or from redundant or uninformative peptides. While the technique is mostly used for smaller, focused studies, there are several reasons to conduct larger targeted experiments. Automated, highly robust software becomes more important in such experiments. In additi
Understanding the driving forces governing protein assembly requires the characterization of interactions at molecular level. We focus on two homologous oppositely charged proteins, lysozyme and alpha-lactalbumin, which can assemble into microspheres. The assembly early steps were characterized through the identification of interacting surfaces monitored at residue level by NMR chemical shift pert
Computer-based design analysis activities are an essential part of most product development projects in industry. An effective integration of the analysis activity into the product development process, especially when the design analysis is performed not by the engineering designer but by an analyst, internal or external to the company, is therefore very valuable. The contribution in this work is
We have compared the amino acid sequence of all four cytosolic sulfotransferases (SULTs) in Drosophila and analyzed their spatial expression patterns during development. Three out of four SULTs show distinct expression activity during embryogenesis, while the 4th SULT shows expression only post-embryonically. st1, st3 and st4 are expressed in non-overlapping expression domains mainly confined to o
This review describes principles and features of thermal biosensors and microbiosensors in flow injection analysis. Examples are given that illustrate the great versatility and excellent operational stability offered by thermal biosensors. The examples are mostly from work with the original type of enzyme thermistor operating with an enzyme column, but there will also be work described involving m
For many years, fatigue-related risk has been recognized as a significant issue for aircraft operators. The International Civil Aviation Organization (ICAO) states that a basic building block underlying the process of managing safety is “sharing safety lessons learned and best practices through the active exchange of safety information.” An example of this organized active exchange is the global f
The clinical outcome following a peripheral nerve injury in the upper extremity is generally better in young children than in teenagers and in adults, but the mechanism behind this difference is unknown. In twenty-eight patients with a complete median nerve injury sustained at the ages of 1-13 years (n=13) and 14-20 years (n=15), the cortical activation during tactile finger stimulation of the inj
This article has two core objectives: first to challenge the conventional understanding of liberal international theory (which we do by focussing specifically on classical liberalism) and second, to develop much further postcolonialism’s conception of Eurocentrism. These twin objectives come together insofar as we argue that classical liberalism does not always stand for anti-imperialism/noninterv
Over the last couple of years, self-organizing nanotubes based on the dipeptide diphenylalanine have received much attention, mainly as possible building blocks for the next generation of biosensors and as drug delivery systems. One of the main reasons for this large interest is that these peptide nanotubes are believed to be very stable both thermally and chemically. Previously, the chemical and
N-CAM, expressed by non-myelinating Schwann cells, was investigated by immunohistochemistry in transected rat femoral nerves, which were repaired either using a straight or a crossed over technique. N-CAM staining covered 30 and 11% of the cross-sectional area of and motor branches, respectively, in uninjured nerves. After 3 days there was a transient smaller area of N-CAM staining following both
