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The N-terminal catalytic module of beta-mannanase TrMan5A from the filamentous fungus Trichoderma reesei is classified into family 5 of glycoside hydrolases. It is further classified in clan A with a (beta/alpha)(8) barrel configuration and has two catalytic glutamates (E169 and E276). It has at least five other residues conserved in family 5. Sequence alignment revealed that an arginine (R171 in
Attempts have been made to make traditional local foods a part of the tourists' experiences, but few have caught great interest among the tourist and leisure consumers. An exception is the Norwegian traditional Sheep's-head meal. This article focuses on driving factors behind this success. Sheep's heads have been continuously available and used at private meals, albeit the status of the meals has
Objective: This randomized methodologic study sought to test the reliability of an Internet questionnaire and investigate the differences in response rates between traditional pen-and-paper questionnaires and Internet questionnaires for measuring patient-reported outcome after total hip replacement surgery. Methods: From the Swedish Hip Arthroplasty Register, 2400 patients were chosen at random bu
Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Ca
Histomorphometry is commonly applied to study bone remodeling. Histological definitions of cortical bone boundaries have not been consistent. In this study, new criteria for specific definition of the transitional zone between the cortical and cancellous bone in the femoral neck were developed. The intra- and inter-observer reproducibility of this method was determined by quantitative histomorphom
The mineral dust cycle responds to climate variations and plays an important role in the climate system by affecting the radiative balance of the atmosphere and modifying biogeochemistry. Polar ice cores provide unique information about deposition of aeolian dust particles transported over long distances. These cores are a palaeoclimate proxy archive of climate variability thousands of years ago.
In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters kcat, Km and product KI for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidas
A powerful alternative to glucose oxidase as anode material in implantable biofuel cells is presented: Cellobiose dehydrogenase (CDH) from the ascomycete Myriococcum thermophilum (MtCDH) catalyzes the electrochemical oxidation of glucose, lactose, and cellobiose over a broad pH range. Current densities of more than 1 mA . cm(-2) can be reached when MtCDH is wired to an Os redox polymer in the pres
1. Hormone-mediated maternal effects and developmental plasticity are important sources of phenotypic variation, with potential consequences for trait evolution. Yet our understanding of the importance of maternal hormones for offspring fitness in natural populations is very limited, particularly in non-avian species. 2. We experimentally elevated yolk testosterone by injection of a physiologica
We report the case of a 58-year-old man with a giant partially thrombosed anterior communicating artery aneurysm, which presented with mass effect. Our treatment strategy consisted of endovascular aneurysm circulatory exclusion prior to surgical resection. To do so, we first occluded both the two A1 segments and the aneurysm neck with a neck-bridge device to prevent further coil migration within t
Aspects of parental interaction were assessed in 20 families with diabetic, insulin-dependent children, using hour-long video-taped interviews, the children being in optimal (O, n = 10) or poor (P, n = 10) metabolic control and showing optimal or poor psychological adaptation. In comparison with the O-group parents, the P-group parents were less appreciative of one another, were less congruent in
New electron density diagnostic line ratios are presented for the O IV 2s(2)2p P-2-2s2p(24)P and S IV 3s(2)3p P-2-3s3p(24)P intercombination lines around 1400 Angstrom comparison of these with observational data for the symbiotic star RR Telescopii (RR Tel), obtained with the Space Telescope Imaging Spectrograph (STIS), reveals generally very good agreement between theory and observation. However
Objectives To report the initial results from Sweden of a large population-based randomized study of screening using prostate-specific antigen (PSA) to detect prostate cancer, as the efficacy of such screening to decrease prostate cancer mortality has not yet been proven. Methods From the population registry men aged 50-66 years were randomized to screening (9973) and to future controls (9973). Me
We present an EBL-defined nanowire pattern that can sort axons coming from different directions on a substrate. The pattern defines tracks for left-bound traffic and right-bound traffic, which opens up new possibilities for designing neural networks on a chip.
The Swedish cue word men ‘but’ can mark the boundary between both different topic units as well as topic-internal units in spontaneous speech. The goal of this study is to see if these two functions of men can be distinguished on the basis of their local prosodic correlates and co-occurring lexical items. Men-tokens in spontaneous narrations were labelled as to their function, first using text-onl
