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To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Funct
Airway basal cells are crucial for regeneration of the human lung airway epithelium, and are thought to be important contributors to Chronic Obstructive Pulmonary Disease (COPD) and other lung disorders. In order to reveal how basal cells contribute to disease, and to discover novel therapeutic targets, these basal cells need to be further characterized. In this study, we optimized a flow cytometr
BACKGROUND: Streptococcus pneumoniae remains a major cause of community-acquired pneumonia, meningitis, and other diseases, contributing significantly to high morbidity and mortality worldwide. Although it responds to antibiotics, their use is becoming limited due to the rise in antibiotic resistance, which necessitates the development of new therapeutics. Nanotechnology is used to counteract anti
Objective To investigate the relationship between active commuting and atherosclerosis in middle age using state-of-the-art coronary CT angiography (CCTA). Methods This cross-sectional study used data from the Swedish CArdioPulmonary bioImage Study (SCAPIS) and included 23 722 randomly recruited individuals aged 50–64 years from six different regions in Sweden. The exposure was commuting mode, ass
Chronic obstructive pulmonary disease (COPD) is a progressive condition characterized by airway remodeling, including emphysema and fibrosis. Proteoglycans and their glycosaminoglycan (GAG) chains are key components of the extracellular matrix and may be altered as the disease advances. This study analyzed lung tissue from COPD patients (GOLD stages II-III and IV), non-COPD smokers, and non-smoker
The production of fast pyrolysis bio-oil (FPBO) from secondary biomass is a promising technique for the production of liquid biofuels. However, the resulting FPBO is often viscous and chemically unstable, requiring significant upgrading while also being difficult to work with. To overcome this issue, FPBO can be hydrotreated using slurry hydrotreatment, which has a long history as a processing tec
This study investigated the presence of microplastics (MPs, >5 μm to <2 mm) in biofertilizers and estimated their mass input to Swedish agricultural soils. Collective samples from representative biogas facilities were analyzed using optical microscopy and optical photothermal infrared (O-PTIR) spectroscopy, with selected MPs examined by scanning electron microscopy (SEM). The highest MPs concentra
Measuring the number concentration of colloidal nanoparticles (NPs) is critical for assessing reproducibility, enabling compliance with regulation, and performing risk assessments of NP-enabled products. For nanomedicines, their number concentration directly relates to their dose. However, the lack of relevant reference materials and established traceable measurement approaches make the validation
Despite a large number of studies, out of all drivers of radiative forcing, the effect of aerosols has the largest uncertainty in global climate model radiative forcing estimates. There have been studies of aerosol optical properties in climate models, but the effects of particle number size distribution need a more thorough inspection. We investigated the trends and seasonality of particle number
BACKGROUND: Spatial epidemiological analyses primarily depend on spatially-indexed medical records. Some countries have devised ways of capturing patient-specific spatial details using ZIP codes, postcodes or personal numbers, which are geocoded. However, for most resource-constrained African countries, the absence of a means to capture patient resident location as well as inexistence of spatial d
BACKGROUND: Streptococcus bovis/equinus complex (SBSEC) comprise several species and subspecies and is a common cause of infective endocarditis (IE). S. gallolyticus subsp. gallolyticus (Sg gallolyticus) accounts for a majority of SBSEC IE, but the risk of IE for other subspecies is largely unknown. We aimed to investigate the clinical presentation of bacteraemia, and proportion of patients with I
We present new estimates of the living standards among the rural labouring classes in Sweden from 1750 to 1900. Starting with a database of more than 1,000 probate inventories of rural, landless and semi-landless people from the years 1750, 1800, 1850 and 1900, we study the development for crofters in particular. In a sub-sample of 120 probate inventories we map in great detail the material items
This study analyzes how risk attitudes influence the agricultural productivity of men and women in a sub- Saharan African country, Burkina Faso. By using a large representative panel survey of farmers, the results show that as female farmers increase risk taking, the productivity of female-owned plots goes down. The study controls for various socio-economic factors and explores how the diversity o
Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on
As production and supply chains rely on exploitative extraction of nature and labour, consumption levels (resource use) remain one of the main indicators of inequality, across and within national contexts. In times of ongoing climate crisis and rising levels of inequalities, welfare systems face the dual challenge of transforming economies to reduce their reliance on unsustainable industries as we
INTRODUCTION: Streptococcus pneumoniae (the pneumococcus) effectively colonizes the human nasopharynx, but can migrate to other host sites, causing infections such as pneumonia and sepsis. Previous studies indicate that pneumococci grown as biofilms have phenotypes of bacteria associated with colonization whereas bacteria released from biofilms in response to changes in the local environment (i.e
Now extinct, the aurochs (Bos primigenius) was a keystone species in prehistoric Eurasian and North African ecosystems, and the progenitor of cattle (Bos taurus), domesticates that have provided people with food and labour for millennia1. Here we analysed 38 ancient genomes and found 4 distinct population ancestries in the aurochs—European, Southwest Asian, North Asian and South Asian—each of whic
