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We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known

In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DN

Amyloid beta peptides (Aβ) proteins play a key role in vascular pathology in Alzheimer’s Disease (AD) including impairment of the blood–brain barrier and aberrant angiogenesis. Although previous work has demonstrated a pro-angiogenic role of Aβ, the exact mechanisms by which amyloid precursor protein (APP) processing and endothelial angiogenic signalling cascades interact in AD remain a largely un

Common remediation and land management approaches often preclude economically attractive and low risk brownfield redevelopment. The authors argue that recently developed dynamic site characterization techniques can greatly improve sound decision making on land revitalization options. A number of these in-situ soil and groundwater test methods, such as MIP, ROST™/UVOST™, XRF and BAT, based on geote

I denna rapport redovisas populationstrender för 208 svenska fågelarter för olika långa tidsperioder. Fåglarna har räknats vinter, vår och sommar enligt strikt standardiserade metoder. Sommarrespektive vinterpunktrutter har räknats sedan 1975, i huvudsak i södra Sverige. Standardrutterna räknas också på sommaren (sedan 1996) men täcker hela Sverige på ett representativt sätt. Nattaktiva fåglar harWe present the results of the Swedish Bird Survey, run by the Department of Biology, Lund University, as a part of the National Monitoring Programme of the Swedish Environmental Protection Agency. The results for 2017 include data from: 575 winter point counts in 2016/2017 (42nd winter), of which 284 were carried out during the Christmas/New Year count, 223 summer point count routes (43rd year) an

4MOST,1 the 4m Multi-Object spectrographic Survey Telescope, is an upcoming optical, fiber-fed, MOS facility for the VISTA telescope at ESO's Cero Paranal Observatory (Chile). The preliminary design of 4MOST features 2,400 fibers split into a low-resolution (1,600 fibers, 390-900 nm, R > 5; 000) and a high-resolution channel (800 fibers, three arms, ∼20-25 nm coverage each, R > 18; 000) with an Ec

We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dy

We present the first one-step acoustic separation of high quality plasma and mononuclear cells (MNCs) from undiluted human whole blood. This enables centrifugation-free integration in analytical instrumentation.

Implementing electrically connected metal-coated posts in a Deterministic Lateral Displacement (DLD) device and applying electric fields, we use electrokinetics to achieve tunable particle separations and to trap and manipulate DNA. The strength of DLD stems from its typically binary behavior. Particles move in one out of two trajectories based on their effective size. For particles that are close

We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing,

In this paper, a distinctly subjectivist analysis of the nature of relational goodness or goodness for is proposed. Like the generic subjectivist analysis of value, the proposal is to analyse value in terms of attitudes. Specifically, the proposed analysis of goodness for appeals to a special kind of attitude: namely, so-called for-someone’s-sake attitudes. Unlike other analyses in the literature

Structural Health Monitoring (SHM) can provide valuable information for maintenance-related activities and post-disaster emergency management. However, as with any technological system, SHM systems can be susceptible to errors due to malfunctioning. Therefore, it is essential to assess the potential for malfunctions and the associated costs of maintenance and repair when evaluating the long-term b

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a

We investigated the impact of inertial forces on acoustic sorting at high sample throughputs. Above a critical flow rate, we observed an unexpected inertia induced breakdown of the sorting efficiency that is not due to the wall induced lift force and was not previously described in the literature. We analyzed the effect experimentally and theoretically, providing an explanation to the phenomena, a

Acquisition of entomological data with high-frequency lidar is an emerging research field in rapid development. The technique offers very high numbers of observations per time unit, suitable for statistical models. In this work, we use a near-infrared Scheimpflug lidar with a sampling frequency of 3.5 kHz to assess the activity of free flying organisms. In-situ measurements were done during the ra