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We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known

In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DN

In this study, we explored the implications of a groundwater development project on women's workload and their experience of marital violence in a Bangladesh village. We believe that the project facilitated irrigation water but also that it resulted in seasonal domestic water shortages. Men used deep motorized pumps for irrigation, and women used shallow handpumps for domestic purposes. Many handp

Based on articles published 1990-2004 in 21 LIS journals, a set of co-citation analyses were performed to study changes in research fronts over the last 15 years, where LIS is at now; and to discuss where it is heading. To study research fronts, here defined as current and influential co-cited papers, a citations among documents methodology was applied; and to study changes, the analyses were time

Popular Abstract in Swedish Dagens storskaliga tillverkning baseras på varor med relativt kort livstid och lågt pris. Vinsterna för tillverkarna kommer från den stora mängden sålda varor. Det finns dock inom många industribranscher krympande vinstmarginaler och många företag söker därför efter nya källor för att uppnå bättre rörelseresultat. Även miljöfrågorna är en utmaning för industrin. VäxandeLife cycle environmental problems have been addressed by a number of strategies. However, the results are mostly lamentable because solutions are searched for within the same paradigms that give rise to the problems. This research questions the vision of linear material growth and searches for ways to sustain economic growth without putting the natural environment under stress. The research explor

Enligt författarnas mångåriga erfarenhet från kustprojekt runt om i Sverige föreligger ett stort behov av en förbättrad terminologi på svenska vad gäller ingenjörsmässiga frågeställningar och planering av kustområden.Föreliggande artikel presenterar således en engelsk-svensk ordlista, inklusive förklarande kommentarer, med syfte att klargöra en lång rad ord och uttryck. Denna finns också att tillg

This work focuses on the investigation on both local and global dielectric properties for a random wound single tooth winding, which represents a segment of the in-field electrical machine used on an Hybrid Wheel Loader. Finite Element Analysis (FEA) is used to predict the temperature stress while loading and illustrate the influences of dielectric properties (i.e. conductivity and dielectric cons

Using Deterministic Lateral Displacement (DLD), we are able to separate bacteria by their size and their chain length. This separation enables the study of these properties as factors of virulence.

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that

A theoretical framework for understanding public library development is presented; plural agonistics is used in combination with an approach inspired by post-humanism. The framework is related to examples from a study of two Swedish public libraries. Antagonism is viewed as foundational, and democracy as an ongoing process, the task being to transform antagonism to agonism, with institutions like

Treatment resistant depression (TRD) and suicidal behavior are among the most important public health problems and are commonly associated with significant disability and psychosocial impairment. Although there have been recent advances in identifying neurobiological correlates of these complex conditions, their pathophysiology still remains unclear. Although the recent advances concerning the neu

Abstract in UndeterminedSometimes energy security is used as an advocacy for renewable energy systems. Although renewable energy systems can surely have such characteristics they will not per se solve all types of security problems, and new problems will most certainly arise. In this paper, energy security aspects of renewable energy systems are analysed taking the starting point in a broad typolo

Kameraövervakning utgör en pågående trend i kommuners och privatpersoners kamp mot brottslighet. Föremålet för denna rapport är ett särskilt fall som sedan april 2004 ansluter till denna trend: övervakningen av parkeringsplatsen på Kasernplan i centrala Landskrona. Rapporten utgår från statistik, medierapportering, enkätundersökningar samt etnografiskt material (intervjuer och fältanteckningar)