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Investigation of the enzyme Bacillus agaradhaerens Cel 5a as an analytical tool in mass spectral characterisation of methylcelluloses
Two methylcelluloses (MC) were investigated by partial hydrolysis followed by direct infusion electrospray ionisation mass spectrometry (ESI-MS). Partial hydrolysis was achieved either enzymatically or by acid hydrolysis. Enzyme hydrolysis was performed using the endoglucanase Bacillus agaradhaerens Cel 5a. Three different hydrolysis buffer solutions were investigated in order to deter-mine which
Yeast "make-accumulate-consume" life strategy evolved as a multi-step process that predates the whole genome duplication.
Prophylaxis and treatment of HIV-1 infection in pregnancy: Swedish recommendations 2007
Prophylaxis and treatment with antiretroviral drugs, a consequent low viral load, and the use of elective Caesarean section, are factors that radically decrease the risk of HIV transmission from mother to child during pregnancy and delivery. The availability of new antiretroviral drugs, updated general treatment guidelines and increasing knowledge of the importance of drug resistance, have necessi
Microglia Acquire Distinct Activation Profiles Depending on the Degree of alpha-Synuclein Neuropathology in a rAAV Based Model of Parkinson's Disease
Post-mortem analysis of brains from Parkinson's disease (PD) patients strongly supports microglia activation and adaptive immunity as factors contributing to disease progression. Such responses may be triggered by alpha-synuclein (alpha-syn), which is known to be the main constituent of the aggregated proteins found in Lewy bodies in the brains of PD patients. To investigate this we used a recombi
Gender and regional differences in perceived job stress across Europe
Background: Over the last 20 years stress at work has been found to be predictive of several conditions such as coronary heart disease, high blood pressure and non- specific sick leave. The Karasek demand/ control/ strain concept has been the most widely used in prospective epidemiological studies. Objectives: To describe distribution in Karasek's demand/ control ( DC) dimensions as well as preval
Understanding and supporting large-scale requirements management
Large market-driven software companies face new challenges in requirements engineering and management that emerged due to their recent extensive growth. At the same time, the pressure generated by competitors’ and users’ expectations demands being more competitive, creative and flexible to more quickly respond to a rapidly changing market situation. In the pursuit of staying competitive in this co
Seasonal modulation of flight speed among nocturnal passerine migrants: differences between short- and long-distance migrants
Migrating birds are expected to fly at higher airspeeds when minimizing time rather than energy costs of their migratory journeys. Spring migration has often been suggested to be more time selected than autumn migration, because of the advantage of early arrival at breeding sites. We have earlier demonstrated that nocturnal passerine migrants fly at higher airspeeds during spring compared to autum
Is there an optimal patent length?
I dagens samhälle är innovation av tilltagande värde för såväl industrialiserade som utvecklingsländer och ett innovativt samhällsklimat innebär ett betydande bidrag till den ekonomiska tillväxten och till samhällsutvecklingen i stort. Det bokförda värdet hos dagens bolag består till en stor och växande del av immateriella tillgångar och det blir ständigt lättare att tillgå mer och bättre kunskap.In today’s society innovation is increasingly important for a developed, as well as a developing, country and it is a significant contributor to economic growth and the evolution of society. The accounted value of companies increasingly consists of immaterial property and our access to existing knowledge is ever greater and ever easier. Since ideas and innovations are of such great importance to m
Tonåringen i dagens samhällsvård : Problematik förknippad med institutionsvård på SiS-hem, familjehemsplacering och insatser inom öppenvården
The purpose of this paper was to establish what possible set of problems might be associated with three different types of social care available to young people. I chose to study institutionalised care, foster care and home-based intervention. Through interviews with three social workers, my aim was to answer the following questions: - What set of problems related to the teenagers tend to generate
Online sexual behaviours among Swedish youth: associations to background factors, behaviours and abuse
Sexual activity online may result in positive experiences for young people, or lead them to engage in risky behaviours possibly resulting in sexual assault or abuse. The aim of our study was to investigate associations between online sexual behaviours among Swedish youth and background factors as well as aspects of wellbeing. The behaviours investigated were: having sex online with a contact met o
Testing for backlash in hiring: A field experiment on agency, communion and gender.
Gender stereotypes describe women as communal and men as agentic. Laboratory-based research (Rudman & Glick, 1999, 2001) suggests that trying to disconfirm such descriptive gender stereotypes (e.g., women self-promoting their agency), entails the risk of hiring discrimination due to violation of prescriptive gender stereotypes: a backlash. To examine whether backlash occurs when applying for r
Size-dependent long-term tissue response to biostable nanowires in the brain.
Nanostructured neural interfaces, comprising nanotubes or nanowires, have the potential to overcome the present hurdles of achieving stable communication with neuronal networks for long periods of time. This would have a strong impact on brain research. However, little information is available on the brain response to implanted high-aspect-ratio nanoparticles, which share morphological similaritie
Sex-Specific Fitness Consequences of Nutrient Intake and the Evolvability of Diet Preferences
The acquisition of nutrients is fundamental for the maintenance of bodily functions, growth, and reproduction in animals. As a result, fitness can be maximized only when animals are able to direct their attention to foods that reflect their current nutritional needs. Despite significant literature documenting the fitness consequences of nutrient composition and preference, less is known about the
A cathodoluminescence study of the influence of the seed particle preparation method on the optical properties of GaAs nanowires.
Cathodoluminescence at 8 K is used to compare the optical properties of AlGaAs-capped GaAs nanowires, grown by metal-organic vapour phase epitaxy and seeded by gold particles prepared by different methods. Six different methods were used to fabricate and deposit gold seed particles onto GaAs substrates: colloid particles, aerosol particles and particles defined by electron beam lithography. The na
A prospective surveillance model for rehabilitation for women with breast cancer
BACKGROUND: The current model of care for individuals with breast cancer focuses on treatment of the disease, followed by ongoing surveillance to detect recurrence. This approach lacks attention to patients' physical and functional well-being. Breast cancer treatment sequelae can lead to physical impairments and functional limitations. Common impairments include pain, fatigue, upper-extremity dy
Blood Cells as a Cellular Biomarker for Mitochondrial Function in a Experimental Model of Acute Carbon Monoxide Poisoning with Treatment
Introduction: Carbon monoxide (CO) is a leading cause of environmental poisoning in the United States with substantial mortality and morbidity. The mechanism of CO poisoning is complex and includes hypoxia, inflammation, and mitochondrial dysfunction. Currently both biomarkers and therapies for CO poisoning are limited and require new approaches. Methods: Rats (~ 300 g) were divided into four grou
Exploring the effect of menstrual loss and dietary habits on iron deficiency in teenagers : A cross-sectional study
Adolescent girls are particularly susceptible to iron deficiency due to increased iron requirements during the pubertal growth spurt, combined with iron loss following menarche. This study aimed to investigate the prevalence of heavy menstrual bleeding in an adolescent population using the SAMANTA questionnaire and to explore its relationship with dietary habits and iron deficiency. This cross-sec
Does the crystal structure of vanadium nitrogenase contain a reaction intermediate? Evidence from quantum refinement
Abstract: Recently, a crystal structure of V-nitrogenase was presented, showing that one of the µ2 sulphide ions in the active site (S2B) is replaced by a lighter atom, suggested to be NH or NH2, i.e. representing a reaction intermediate. Moreover, a sulphur atom is found 7 Å from the S2B site, suggested to represent a storage site for this ion when it is displaced. We have re-evaluated this struc
Adsorption of Fibrinogen on Silica Surfaces-The Effect of Attached Nanoparticles
When a biomaterial is inserted into the body, proteins rapidly adsorb onto its surface, creating a conditioning protein film that functions as a link between the implant and adhering cells. Depending on the nano-roughness of the surface, proteins will adsorb in different amounts, with different conformations and orientations, possibly affecting the subsequent attachment of cells to the surface. Th
