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Human Fetal Hemoglobin : Biochemical Characterization and Recombinant Production

Popular Abstract in English Blood, a deep red fluid, circulates in vessels throughout our body. It consists of two main components, a liquid part and a cell part. The liquid portion, which is also called ‘plasma’, takes a main function in transporting water, nutrients, and immune substances. The cell components, which are suspended in the plasma, consist of three main cell types including plateletBlood transfusion is a common medical procedure when oxygen supply in the body is impaired. However, blood transfusion is not always without risks. The uncontrolled spreading of blood borne pathogens such as HIV or hepatitis B and C virus has urged researchers to look for “blood substitutes”. Many research groups have put their focuses on hemoglobin-based products (HBOCs). The major source of haem

Portal Stability Controls Dynamics of DNA Ejection from Phage

Through a unique combination of time-resolved single-molecule (cryo-TEM) and bulk measurements (light scattering and small-angle X-ray scattering), we provide a detailed study of the dynamics of stochastic DNA ejection events from phage λ. We reveal that both binding with the specific phage receptor, LamB, and thermo-mechanical destabilization of the portal vertex on the capsid are required for in

Separate versus simultaneous saccharification and fermentation of two-step steam pretreated softwood for ethanol production

In two previous studies, optimal conditions were identified for two-step steam pretreatment Of SO2- and H2SO4-impregnated softwood. In the present study the yield of sugar and ethanol was determined in a process development unit where pretreatment was performed in a 10-L reactor and simultaneous sacchatification and fermentation (SSF) or enzymatic hydrolysis (EH) were performed in 30-L reactors. T

Which cancer patients die in nursing homes? Quality of life, medical and sociodemographic characteristics

In this study, cancer patients' characteristics associated with death in nursing homes were explored. The study sample included 395 cancer patients who had participated in a trial of palliative care, 260 ( 66%) patients died in hospital, 80 (20%) at home and 55 (14%) in nursing homes. Health-related quality of life was prospectively recorded using the EORTC QLQ-C30 questionnaire. Death in nursing

Prevalence, awareness, treatment, and control of hypertension: Rule of thirds in the Skaraborg project

Objective. To describe the prevalence, awareness, and control of hypertension in a Swedish population during the early 2000s to address implications for care and prevention. Design. A cross-sectional population survey. Setting. Primary health care in Skaraborg, a rural part of western Sweden. Subjects. Participants (n = 2816) in a population survey of a random sample of men and women between 30 an

Chromosome banding analysis of cells from fine-needle aspiration biopsy samples from soft tissue and bone tumors: is it clinically meaningful?

Morphologic evaluation of samples from fine-needle aspiration (FNA) and core needle (CN) biopsies is an important part of the pretreatment diagnosis of bone and soft tissue tumors. Because most such tumors have characteristic, sometimes even specific, chromosomal rearrangements, ancillary genetic analyses could provide important diagnostic information. Whereas directed analyses, such as fluorescen

Development and Psychometric Evaluation of a New Instrument for Measuring Sleep Length and Television and Computer Habits of Swedish School-Age Children.

The aim was to develop a new instrument for measuring length of sleep as well as television and computer habits in school-age children. A questionnaire was constructed for use when children visit the school health care unit. Three aspects of the validity of the questionnaire were examined: its face validity, content validity, and construct validity. Test-retest reliability was assessed by giving t

Nano- and Micro-sized Molecularly Imprinted Polymer Particles on Solid Surfaces

Popular Abstract in English Nanoscience and nanotechnology have existed in this world before scientists actually coined the terms. Nature around us and human beings themselves are all made of nanomaterials (1-100 nm), such as DNA, proteins, liposomes, and enzymes. These building blocks possess molecular recognition properties, resulting in their self-assembly to form various two- and three-dimensiMolecularly imprinted polymers (MIPs) are artificial receptors made by imprinting template molecules in a polymer matrix followed by their removal through washing to obtain a specific and selective template cavities. This property of the MIPs have made them a very efficient material for diverse applications such as chromatography, purification, drug sensing, etc. Recently, zero-dimensional polymer

Design process for innovation in pharmaceutical packaging: reviewing needs and modes of activity

The innovation process is a challenge for companies trying to achieve better outcomes through new ideas or products. The related literature discusses the importance of innovation as a learning process, underlined by a design perspective. Supported by design, the innovation process combines multidisciplinary aspects of creativity and dynamism, mainly for end-user oriented industries. For other indu

Structure and mobility in water plasticized poly(ethylene oxide)

The change in structure and mobility of poly(ethylene oxide) (PEO) containing 2 wt% of fumed silica and the water self-diffusion coefficient in concentrated PEO-water systems have been investigated at room temperature in the water weight fraction, w(w) range 0-0.50 w/w. Pulsed field gradient nuclear magnetic resonance was used to measure the self-diffusion coefficients. Structure and mobility prop

Clinical impact of breakpoint position within M-bcr in chronic myeloid leukemia

We have analyzed the M-bcr breakpoint position in 133 Philadelphia-positive chronic myeloid leukemia patients and correlated the findings with clinical, hematologic, and cytogenetic data. We also investigated the splicing pattern of the BCR-ABL mRNA in 30 patients, using reverse transcriptase PCR. No statistically significant differences were found between breakpoint position within M-bcr and clin

Tender or not tender: Test-retest repeatability of pressure pain thresholds in the trapezius and deltoid muscles of healthy women

Objective: To examine the test-retest repeatability of pressure pain thresholds in the shoulder muscles of healthy women. Design: Four experimental sessions (days 1, 3, 28, 30), each including 4 consecutive pressure pain threshold measurements at 10-minute intervals. Subjects: Twenty-four healthy female volunteers, mean age 42 years. Methods: Two examiners measured pressure pain thresholds bilater

Recent changes in land use and productivity in agro-pastoral Inner Mongolia, China

Popular Abstract in Swedish De arida och semi-arida grässtäpperna i Norra Kina anses vara svårt drabbade av markförstöring, främst genom vind- och vattenerosion, men även genom försaltning. Den rådande uppfattningen är att markförstöring och ökenspridningsproblematiken har eskalerat sedan införandet av de ekonomiska reformerna efter 1978, bl.a. genom en expansion av den odlade arealen på tidigare This study challenges the prevailing assumption that the expansion of cultivated land areas and increasing number of livestock in the agro-pastoral regions of northern China have aggravated the process of land degradation since the start of the rural reforms in 1978. Land-use and productivity trends in the Inner Mongolia Autonomous Region (IMAR), with special attention to the Keerqin steppe region

Force response to rapid length change during contraction and rigor in skinned smooth muscle of guinea-pig taenia coli

1. Mechanical transients in fibre bundles of skinned smooth muscle of guinea-pig taenia coli at 21-22 degrees C were investigated by recording tension responses to length changes of up to 9%, complete within 0.3 ms. 2. The length-force relationship, recorded continuously during rapid stretch of a Ca(2+)-activated contracted muscle, was linear up to at least 2.5 times the isometric force, correspon

The Swedish knee arthroplasty register. A nation-wide study of 30,003 knees 1976-1992

1976 through 1992, 30,003 primary knee arthroplasties and their revisions have been recorded in a nation-wide Swedish study. We report on the structure of the register, demographic data and survivorship. We found that operations for osteoarthrosis (OA) counted for the increase in number of arthroplasties in contrast to rheumatoid arthritis (RA), where the number had slightly declined. For primary

The Welfare State or the Economy? Preferences, Constituencies, and Strategies for retrenchment

The assumption that voters systematically defend the welfare state is challenged by recent research showing that parties are on average not punished and sometimes even rewarded for welfare state retrenchment. We work to understand better the micro-foundations for this finding of non-punishment by exploring individuals’ preferences over social policy. In particular, we distinguish general support f

Genetic variation in FADS genes is associated with maternal long-chain PUFA status but not with cognitive development of infants in a high fish-eating observational study.

Long-chain n-6 and n-3 PUFA (LC-PUFA), arachidonic acid (AA) (20:4n-6) and DHA (22:6n-3), are critical for optimal brain development. These fatty acids can be consumed directly from the diet, or synthesized endogenously from precursor PUFA by Δ-5 (encoded by FADS1) and Δ-6 desaturases (encoded by FADS2). The aim of this study was to determine the potential importance of maternal genetic variabilit

Assessment of Body Composition in Subjects with Metabolic Syndrome Comparing Single-Frequency Bioelectrical Impedance Analysis and Bioelectrical Spectroscopy.

Abstract Background: The aim of this study was to investigate the agreement between body composition measurements made with two methods-single-frequency bioelectrical impedance analysis (SF-BIA) and bioelectrical impedance spectroscopy (BIS). Methods: The body composition measurements using SF-BIA and BIS were performed seven times during 6 months on 41 patients (13 men and 28 women) with metaboli