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Ideals and visions of a Library 2.0 have in recent years been widely discussed in public library research and practice. Influenced by the Web 2.0 discourse, the Library 2.0 rhetoric has to a large extent been coloured by utopian and revolutionary overtones, identifying social media as key to transforming the public library institution. While previous research has primarily addressed this developme
Northern Europe is in the forest zone, but wild megaherbivores have maintained grass-dominated vegetation here for the last 1.8 million years. Continuity of the grassland biome through glacialinterglacial cycles and connection to steppe vegetation has resulted in the evolution, immigration, and survival of a large number of grassland species. During the last millennia the effect of wild ungulates
This chapter will look at why, how and in what way a spontaneous memorial may develop: from an immediate act to a more planned place of grief and remembrance (Clark and Franzmann 2006, Nieminen Kristofersson 2006, Klaasens, Groote and Huigen 2009). Of special importance for this investigation are the noticeable differences in attitudes to and growths of roadside memorials revealed by data gleaned
Lattice Boltzmann method (LBM) is a method that can be used to capture the detailed activities of the transport processes at microscale. Here LBM is used to model the porous anode for an anode-supported Solid Oxide Fuel Cell (SOFC). The purpose of this study is to investigate the effects of electrochemical reactions on the transport processes by a 3D model at microscale. A porous 3D modeling domai
Popular Abstract in Swedish Neutrofilen är den vanligaste vita blodkroppen och de tillhör det sk medfödda immunförsvaret. Under normala förhållanden är neutrofilerna inaktiva och cirkulerar i blodomloppet. Vid exempelvis en infektion utsöndras inflammatoriska aktiverande ämnen från vår kropp men även från oönskade mikroorganismer och detta gör att neutrofilerna aktiveras. Från att ha varit runda, Inflammation is a localized protective response in vascularized tissues and can be induced by microbial infection or cell and tissue injury. Polymorphonuclear neutrophils (PMN) are the most common white blood cells and are recognized as major cellular mediators of acute inflammation. PMN are highly mobile cells that possess regulated mechanisms for controlling the expression and activation of adhe
This thesis describes the affinity and enantiomeric selectivity of the cellobiohydrolase Cel7A (isolated from Hypocrea jercorina). The chiral recognition of the enzyme has been studied with respect to amino alcohols and new ligands have been designed, based on the structure of the common ?-blocker propranolol. Capillary electrophoresis have been used to determine the dissociation constants of the
The research described in this thesis has an interdisciplinary approach dealing with chemical, biological, and technical issues. One objective was to interface miniaturized liquid chromatography (LC) to mass spectrometry, i.e. MALDI-TOF MS and ESI-MS, in an unattended mode for high sensitivity determination of proteins and peptides. The coupling between capillary LC and MALDI was accomplished by e
The construction sector provides the direct means to the development and expansion of economic activities, but is at the same time a major consumer of physical and natural resources and a polluter of the environment. Over the last decades, the environmental impact of construction activities has grown dramatically due to the increase in the world population and greater industrial and human activity
The p53 tumor suppressor gene regulates growth arrest and apoptosis after DNA damage. Recent studies suggest that p53 is inactive in vascular smooth muscle cells (VSMCs) in human angioplasty restenosis, promoting VSMC accumulation and vessel stenosis. In contrast, the success of irradiation (brachytherapy) for in-stent restenosis argues that DNA-damage p53 responses are intact. We examined p53 exp
SoPIP2;1 is one of the major integral proteins in spinach leaf plasma membranes. In the Xenopus oocyte expression system its water channel activity is regulated by phosphorylation at the C terminus and in the first cytosolic loop. To assess its structure, SoPIP2;1 was heterologously expressed in Pichia pastoris as a His-tagged protein and in the non-tagged form. Both forms were reconstituted into
α-Galactosidase (EC 3.2.1.22) and β-mannosidase (EC 3.2.1.25) participate in the hydrolysis of complex plant saccharides such as galacto(gluco)mannans. Here we report on the cloning and characterization of genes encoding an α-galactosidase (AglC) and a β-mannosidase (MndA) from Aspergillus niger. The aglC and mndA genes code for 747 and 931 amino acids, respectively, including the eukaryotic signa
Hepatitis C virus (HCV) belongs to the Hepacivirus genus in the Flaviviridae family. Among the least known viral proteins in this family is the nonstructural protein NS4B, which has been suggested to be a part of the replication complex. Hydrophobicity plots indicate a common profile among the NS4B proteins from different members of the Flaviviridae family, suggesting a common function. In order t
TECK (thymus-expressed chemokine), a recently described CC chemokine expressed in thymus and small intestine, was found to mediate chemotaxis of human G protein-coupled receptor GPR-9-6/L1.2 transfectants. This activity was blocked by anti-GPR-9-6 monoclonal antibody (mAb) 3C3. GPR-9-6 is expressed on a subset of memory alpha4beta7(high) intestinal trafficking CD4 and CD8 lymphocytes. In addition,
Magnesium chelatase is the first unique enzyme of the bacteriochlorophyll biosynthetic pathway. It consists of three subunits (BchI, BchD, and BchH). Amino acid sequence analysis of the Rhodobacter capsulatus BchH revealed a novel cysteine motif ((CX2CX3CX14C)-C-393) that was found in only six other proteobacteria (CX2CX3CX11-14C). The cysteine motif is likely to coordinate an unprecedented [Fe-S]
The fragmentation of 23S rRNA of 23 Haemophilus influenzae strains and eight strains belonging to other Haemophilus species was investigated. Instead of intact molecules, the 23S rRNA molecules were found to be cleaved into two to five smaller conserved fragments in most strains examined, especially in H. influenzae type b (5/6) and nontypeable strains (5/5). One or two conserved potential cleavag
