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Protein deletions are frequent among both disease-causing and tolerated variants. Several mechanisms at the DNA, RNA and protein levels can lead to deletions. Many deletions are misclassified in the literature and databases, especially when the mRNA is degraded by the cellular quality-control mechanism. We developed a novel predictor for sequence retaining protein deletions, i.e., variants that do
Zirconium hydride is commonly used for next-generation reactor designs due to its excellent hydrogen retention capacity at temperatures below 1000 K. These types of reactors operate at thermal neutron energies and require accurate representation of thermal scattering laws (TSLs) to optimize moderator performance and evaluate the safety indicators for reactor design. In this work, we present an ato
A significant surface wettability transition from hydrophobicity to hydrophilicity after long-term operation is observed in graphite bipolar plates of a proton exchange membrane fuel cell (PEMFC). Extensive in-situ electrochemical characterization was performed to investigate the impact of bipolar plate wettability on the performance and durability of two PEMFCs (named “New cell” and “Old cell”),
Complement activation protects against infection but also contributes to pathological mechanisms in a range of clinical conditions such as autoimmune diseases and transplant rejection. Complement-inhibitory drugs, either approved or in development, usually act systemically, thereby increasing the risk for infections. We therefore envisioned a novel class of bispecific antibodies (bsAbs) which are
Atmospheric new particle formation (NPF) is a naturally occurring phenomenon, during which high concentrations of sub-10 nm particles are created through gas to particle conversion. The NPF is observed in multiple environments around the world. Although it has observable influence onto annual total and ultrafine particle number concentrations (PNC and UFP, respectively), only limited epidemiologic
Birds fly with different objectives at different times of the annual cycle. For example, when migrating the objective may be to minimize energy cost per unit distance covered or increase the maximum overall speed of migration. A foraging bird flying between food patches may choose to fly at a speed that maximizes the overall food intake rate, while during a display flight the objective is likely t
During human embryogenesis, distinct waves of hematopoiesis give rise to various blood cell types, originating from hemogenic endothelial (HE) cells. As HE cells reside in hypoxic conditions in the embryo, we investigated the role of hypoxia in human endothelial to hematopoietic transition and subsequent hematopoiesis. Using single-cell RNA sequencing, we describe hypoxia-related transcriptional c
Lytic polysaccharide monooxygenases (LPMOs) form a copper-dependent family of enzymes classified under the auxiliary activity (AA) superfamily. The LPMOs are known for their boosting of polysaccharide degradation through oxidation of the glycosidic bonds that link the monosaccharide subunits. This oxidation has been proposed to be dependent on either O2 or H2O2 as cosubstrate. Theoretical investig
The nucleotide sequence coding for the beta chain of murine C3 was determined from cloned cDNA and genomic DNA fragments. Sonicated subfragments were randomly inserted into the bacteriophage M13 and sequenced using the dideoxynucleotide technique. Each nucleotide was sequenced on average six times in these studies. The derived amino acid sequence includes a signal peptide and a tetra-arginine sequ
Arsenic is a toxic metalloid which is found all over the globe. Due to its toxicity and wide abundance, all living organisms have evolved intrinsic arsenic resistance systems. In this study, three proteins that provide arsenic resistance in the Gram-positive bacterium Bacillus subtilis have been investigated. Acr3 is a trans-membrane protein that extrudes arsenite to the cell exterior. It belongs
Popular Abstract in Swedish Många industridesignkonsultföretag har som ambition att anta en strategisk roll i sina kundföretag. Design ses i detta sammanhang som ett viktigt konkurrensmedel med en kreativ process som stödjer en innovationsdriven verksamhet. Samtidigt saknas ofta kunskap, framförallt i små och medelstora företag - SMF, om hur designmetoder och processer kan bidra utöver skapandet aWhile the design industry is moving into new domains, it seems that potential customers do not always understand how the designer can contribute beyond the aesthetically appealing product. The overall purpose of this thesis is to expand our understanding of design as an enabling service in the context of small and medium sized enterprises. Enabling design services have the potential to result in o
Aquaporins (AQPs), membrane proteins responsible for facilitating water transport, found in plant membrane vesicles (MV), have been related to the functionality and stability of MV. We focused on AQPs obtained from broccoli, as they show potential for biotechnological applications. To gain further insight into the role of AQPs in MV, we describe the heterologous overexpression of two broccoli AQPs
Pollutions on and within the underground poses risks for groundwater contamination and is a widespread global problem. Common remediation methods based on digging and removal can be expensive and have limitations, while in-situ remediation is an attractive alternative. However, there is a need to develop tools to monitor the effectiveness both in terms of the successful injection of remediation fl
Outcrops of the Ordovician System in South Africa are extensive; they cover significant portions of the Northern, Western and Eastern Cape provinces as part of the Cape Fold Belt as well as the KwaZulu-Natal Province as supracrustal cover overlying the Natal sector of the Paleoproterozoic Namaqua-Natal metamorphic province. Within the Cape Fold Belt, Ordovician rocks of the Table Mountain Group (P
Harp seals (Phoca groenlandicus), once present in the Baltic Sea, now stand extinct. During the Middle Neolithic period, they held significant dietary importance for the Pitted Ware Culture hunter-fisher-gatherers in Scandinavia. Because they are no longer available for ecological studies, little is known about their specific behavioural intricacies, such as diet, mobility, and reproductive strate
Revealing the local structure information of relaxor ferroelectrics is necessary for a clear understanding of their structure-property relationships, especially the determination of the size of polar nanoregions (PNRs), which is still a long-standing challenge. In this work, the local structure of the pseudo-cubic solid solutions 0.60Bi(Mg1/2Ti1/2)O3-0.40PbTiO3 and 0.65Bi(Mg1/2Ti1/2)O3-0.35PbTiO3,
