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Genome-wide association studies (GWAS) have long relied on proposed statistical significance thresholds to be able to differentiate true positives from false positives. Although the genome-wide significance P-value threshold of 5 × 10(-8) has become a standard for common-variant GWAS, it has not been updated to cope with the lower allele frequency spectrum used in many recent array-based GWAS stud
Tyr-762 is an autophosphorylation site in the human platelet-derived growth factor (PDGF) alpha-receptor. In order to investigate whether phosphorylated Tyr-762 serves as a docking site for downstream signal transduction molecules, affinity purification using an immobilized synthetic peptide containing phosphorylated Tyr-762 and its surrounding amino acid residues was performed. Proteins in HeLa c
Background. To characterize participant reasons for withdrawing from a diabetes focused longitudinal clinical observational trial (TEDDY) during the first three study years. Methods. 8677 children were recruited into the TEDDY study. At participant withdrawal staff recorded any reason parents provided for withdrawal. Reasons were categorized into (1) family characteristics and (2) protocol reasons
POU5F1 has two alternatively spliced transcripts: a long (variant 1, NM_002701) and a short (variant 2, NM_203289) transcript. Only variant 1 is a key regulator of pluripotency. Hence, it is important to be able to distinguish this transcript from variant 2 and from the many pseudogenes present in the genome. Previous studies on the expression of POU5F1 were, however, usually carried out without c
Angiogenesis is a key event in cancer progression and therefore a promising target in cancer treatment. Galectin-1, a β-galactoside binding lectin, is up-regulated in the endothelium of tumors of different origin and has been shown to be the target for anginex, a powerful anti-angiogenic peptide with anti-tumor activity. Here we show that when bound to anginex, galectin-1 binds various glycoprotei
The Variation Ontology (VariO) is used for describing and annotating types, effects, consequences and mechanisms of variations. To facilitate easy and consistent annotations, the online application VariOtator was developed. For variation type annotations VariOtator is fully automated, accepting variant descriptions in Human Genome Variation Society (HGVS) format, and generating VariO terms, either
Although the direction of loads applied to the proximal human femur is unpredictable during sideways fall, most in vitro and numerical simulations refer to a single loading condition (15° internal rotation; 10° adduction), which has been anecdotally suggested in the 1950s. The aim of the present study was to improve in vitro simulations of sideways falls on the proximal femur. An in vitro setup w
Titanium dioxide (TiO2) has very high affinity for phosphopeptides and in recent years it has become one of the most popular methods for phosphopeptide enrichment from complex biological samples. Peptide loading onto TiO2 resin in a highly acidic environment in the presence of 2,5-dihydroxybenzoic acid (DHB), phthalic acid, lactic acid, or glycolic acid has been shown to improve selectivity signif
Colophony is not used as a fragrance ingredient. However, colophony is a well-established marker of fragrance contact allergy(2-4) . Colophony was therefore included as a fragrance allergy marker in our recent paper in BJD on contact allergy in the general population (prevalence 1.0%; 95%-CI 0.6%-1.3%)(5) . When including colophony as a fragrance marker in the article on fragrance contact allergy(
This paper investigates how & to what degree English is used in specific elite domains in Sweden through analysis of (1) language requirements to participate in undergraduate & graduate programs at competitive institutions, & (2) language qualifications & language use patterns in elite professions. We find that English is used pervasively, but not exclusively. The paper also explor
In this correspondence the error exponent and the decoding complexity of binary woven convolutional codes with outer warp and with binary convolutional codes as outer and inner codes are studied. It is shown that an error probability that is exponentially decreasing with the product of the outer and inner code memories can be achieved with a nonexponentially increasing decoding complexity
The nonreceptor tyrosine kinase Src binds to and is activated by the beta-receptor for platelet-derived growth factor (PDGF). The interaction leads to Src phosphorylation of Tyr934 in the kinase domain of the receptor. In the course of the functional characterization of this phosphorylation, we noticed that components of 136 and 97 kDa bound to a peptide from this region of the receptor in a phosp
It is demonstrated that an accurate approximation of the screening function is crucial for a quantitative evaluation of the XPS asymmetry index α. It is also shown that the dynamical aspects of the valence electron response are important in order to get an accurate description of the XPS spectrum at finite distances from the threshold.(23 refs)
BACKGROUND: Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures of correlates of protection. To address this, an international collaboration (NeutNet) involving 18 independent participants was organized to compare different assays. METHODS: Ea
The serum metabolome contains a plethora of biomarkers and causative agents of various diseases, some of which are endogenously produced and some that have been taken up from the environment1. The origins of specific compounds are known, including metabolites that are highly heritable2,3, or those that are influenced by the gut microbiome4, by lifestyle choices such as smoking5, or by diet6. Howev
How can anthropologists negotiate access in high-profile, bureaucratic apparatuses, such as a UN human rights monitoring mechanism – and what can a detailed account of these negotiations tell us of such apparatuses, their operational dynamics and the processes through which they exert an impact, broadly construed? This article addresses these questions through the notion of tactical subjectivity b
In February, 2020, the European Commission published a white paper on artificial intelligence (AI) as well as an accompanying communication and report. The paper sets out policy options to facilitate a secure and trustworthy development of AI and considers health to be one of its most important areas of application. We illustrate that the European Commission's approach, as applied to medical AI, p
This chapter presents the theory of ecologically unequal exchange (EUE). The theory explains asymmetric transfers of non-monetary, biophysical resources and environmental harms between different countries or regions. The chapter provides a cursory review of the literature on methods for empirically identifying such asymmetries and testing hypotheses derived from the theory. To identify EUE require
Nano-ceramic particles can serve as reinforcing agents for metallic materials to improve their mechanical properties. However, it is important to ensure chemical compatibility between the matrix and particles. In the present study, magnesium composites with and without nano-hydroxyapatite (nHA) particles were fabricated for bone reconstruction applications. Two different techniques were used, Conv
