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11fc6802

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https://www.lu.se/sites/www.lu.se/files/11fc6802.pdf - 2026-04-29

Quantification of oligonucleotide modifications of small unilamellar lipid vesicles

We present a new method for quantification of the coupling efficiency between amphiphilic oligonucleotides and suspended small unilamellar lipid vesicles (SUVs). The method employs a supported (phospho) lipid bilayer (SLB)-modified sensor template, which upon exposure to a mixture of SUVs and amphiphilic DNA reacts neither with free SUVs nor with DNA-modified SUVs, but with free DNA only. Using ca