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Mycoplasma bovis (M. bovis) infections are difficult to diagnose and treat, causing severe economic losses to farms with infected cows. Eurofins Milk Testing Sweden AB screens cow’s milk using qPCR (quantitative Polymerase Chain Reaction) to detect M. bovis infections. Five performance characteristics were chosen to evaluate if ELISA (Enzyme-Linked Immunosorbent Assay) could replace the current sc
Methylation of lysine (Kme) is known to regulate several important functions, altering gene expression and cell cycle progression. However, mass spectrometry based proteomic analysis of the modification has not been possible due to the lack of efficient purification strategies to enrich modified peptides for analysis. In this study, we identified and evaluated several in-house and commercial candi
With protectionism on a global rise, non-tariff measures have been an object for public debates and research, as the work of WTO has reduced the possibilities of raising traditional trade restrictions as customs tariffs. Hence governments have turned to non-tariff measures and regulatory standards to fulfill their agendas. This study aims at shedding light on the question of whether non-tariff mea
The demand for high data rates in a wireless communication system has experienced a remarkable growth over the last years. This demand is continually increasing with the growing number of wirelessly connected devices. Thus, there is a continuing hunt for increasing the data transmission rate in wireless communication systems. To increase the data rate, the modern wireless communication system cann
There is a theoretical debate among social movement scholars around an increasing concern with the role of technology in terms of key social movement activities, including mobilization, running and engaging in campaigns and making claims on authorities. This research deals with this debate and contributes to it with this case study on the Georgian women’s movement. The research examines women’s m
During the last decades, the construction industry has struggled with negative press concerning an unethical work environment and inadequate management. The unethical work environment leads to unsatisfied employees, and both internal and external activities are rising to solve this problem. There are limited research understanding of how an ethical climate and ethical leadership are associated to
This thesis aims at defining the improvement areas for packaging and logistics of a long shelf-life Danone Nutricia aseptic product. By literature research and interview, the author is able to build a product mapping protocol to define the sensitive components requiring particular concerns on packaging and logistics. This protocol could be implemented in both academic researches and industry proje
Industrialization has been the key engine for economic growth in the developed world, and currently these economies are substituting away from manufacturing towards services. The alarming trend is that developing countries are doing so as well to an even greater extent and at a lower level of GDP and development. This phenomenon of deindustrialization is premature for developing economies as they
Histomorphometry is commonly applied to study bone remodeling. Histological definitions of cortical bone boundaries have not been consistent. In this study, new criteria for specific definition of the transitional zone between the cortical and cancellous bone in the femoral neck were developed. The intra- and inter-observer reproducibility of this method was determined by quantitative histomorphom
The mineral dust cycle responds to climate variations and plays an important role in the climate system by affecting the radiative balance of the atmosphere and modifying biogeochemistry. Polar ice cores provide unique information about deposition of aeolian dust particles transported over long distances. These cores are a palaeoclimate proxy archive of climate variability thousands of years ago.
In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters kcat, Km and product KI for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidas
Abstract in UndeterminedA method for solving the change in capacitance (or charge) if an object is introduced in a parallel plate capacitor is developed. The integral representation of the potential is exploited in a systematic way to solve the potential everywhere inside the capacitor. In particular, the change in capacitance is extracted. The method shows similarities with the null field approac
In this study, different flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenases (FADGDHs) were characterized electrochemically after "wiring" them with an osmium redox polymer [Os(4,4'-dimethyl-2,2'-bipyridine)(2)(PVI)(10)Cl](+) on graphite electrodes. One tested FADGDH was that recently discovered in Glomerella cingulata (GcGDH), another was the recombinant form expressed in Pichia pa
We use a combination of different scattering techniques and rheology to highlight the link between structure and dynamics of dense aqueous suspensions of soft repulsive colloids in the vicinity of a glass transition. Three different latex formulations with an increasing amount of the hydrophilic component resulting in either purely electrostatically or electrosterically stabilized suspensions are
With diversified requirements and varying manufacturing environments, the optimal production planning for a steelmill becomes more flexible and complicated. The flexibility provides operators with auxiliary requirements through an implementable integrated production planning. In this paper, a mixed-integer nonlinear programming (MINLP) model is proposed for the optimal planning that incorporates v
The design update of the European Spallation Source (ESS) accelerator is almost complete and the construction of the prototype of the microwave discharge ion source able to provide a proton beam current larger than 70 mA to the 3.6 MeV Radio Frequency Quadrupole (RFQ) started. The source named PS-ESS (Proton Source for ESS) was designed with a flexible magnetic system and an extraction system able
Milk coagulation is an important processing trait, being the basis for production of both cheese and fermented products. There is interest in including technological properties of these products in the breeding goal for dairy cattle. The aim of the present study was therefore to estimate genetic parameters for milk coagulation properties, including both rennet- and acid-induced coagulation, in Swe
Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key
