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Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbach
The xyn1 encoded 5 domain xylanase from the thermophilic bacterium Rhodothermus marinus binds specifically to xylan, β-glucan and amorphous but not crystalline cellulose. Our results show that the binding is mediated by the full length xylanase, but not by the catalytic domain only. Based on similarities concerning both predicted secondary structure and binding specificity found with one cellulose
Viral etiology of nasal polyps was postulated as many as 40 years ago, but so far, no study has shown an association or causal relation between any specific virus and nasal polyps. By using the polymerase chain reaction (PCR) technique, nasal polyps from both 10 patients with intolerance to nonsteroidal anti-inflammatory drugs (NSAID intolerance) (e.g., Aspirin) and from 10 patients with no histor
Few empirical studies have investigated career‐related incentives for party membership, including for young party members. Through the lens of rational choice, we ask what career‐related incentives young and active party members consider when calculating costs and benefits of party membership. We argue for a broad understanding of career‐related incentives, including careers outside party politics
Patients diagnosed with chromosome microdeletions or duplications, known as copy number variants (CNVs), present a unique opportunity to investigate the relationship between patient genotype and cell phenotype. CNVs have high genetic penetrance and give a good correlation between gene locus and patient clinical phenotype. This is especially effective for the study of patients with neurodevelopment
The utilization of neural stem cells and their progeny in applications such as disease modelling, drug screening or safety assessment will require the development of robust methods for consistent, high quality uniform cell production. Previously, we described the generation of adherent, homogeneous, non-immortalized mouse and human neural stem cells derived from both brain tissue and pluripotent e
Settings in social work may be associated with spatial stigma. This study uses the case of a highly stigmatised setting to investigate ways of ascribing positive characteristics to tainted contexts. Guided by symbolic interactionism, the aim is to analyse how residents in ‘wet’ eldercare facilities manage to view these in a positive light. Wet eldercare facilities are designed for older people wit
Current sustainability assessment (SA) tools to help deal with contaminated groundwater sites are inherently subjective and hardly applied. One reason may be lack of proper tools for addressing contaminant spread which are basically objective. To fill this gap, there is a need for contaminant transport models that provide site managers with needed room for applying their judgments and consideratio
The fifth generation (5G) of mobile communications introduces improvements on many fronts when compared to its previous generations. Besides the performance enhancements and new advances in radio technologies, it also integrates other technological domains, such as cloud-to-things continuum and artificial intelligence. In this work, the 5G-DIVE Elastic Edge Platform (DEEP) is proposed as the linki
This report will discuss the influence of research into superfluid turbulence on my composition Der Rufer for percussion quartet. Written for Professor Olaf Tzschoppe, director of the Bremen Percussion Quartet, this work is the first result of a planned long-term collaboration with J.G. Weisend II, a Deputy Head of Accelerator Projects at the European Spallation Source (E.S.S.).
An on-board measurement system has been developed that in real time identifies the probability for occurrence and also the exact location of severe wear in the wheelrail contact. Noise generated by the wheel-rail contact is a troublesome side effect both when railway vehicles negotiate curves and run on straight tracks. The concept behind this project is to use this noise as an indicator of the tr
An economic model for determining the optimal automation level for discrete batch manufacturing is presented. The model describes how the costs of parts varies between manufacturing systems of different types at differing automation levels, the part costs often being highly dependent upon the automation level selected. The optimal automation level in a given case can be determined using a relative
Accurate delineation of organs at risk (OAR) is a crucial step in radiation therapy (RT) treatment planning but is a manual and time-consuming process. Deep learning-based methods have shown promising results for medical image segmentation and can be used to accelerate this task. Nevertheless, it is rarely applied to complex structures found in the pelvis region, where manual segmentation can be d
Isolation of DNA from blood and buccal swabs in adequate quantities is an integral part of forensic research and analysis. The present study was performed to determine the quality and the quantity of DNA extracted from four commonly available samples and to estimate the time duration of the ensuing PCR amplification. Here, we demonstrate that hair and urine samples can also become an alternate sou
The last decade and a half has seen intense research efforts into metal-halide perovskite (MHP) materials, as researchers have come to appreciate their excellent photo-electrical properties such as good radiative recombination rates and light absorption. As part of these efforts MHP-devices of different morphologies have been developed. Notably the enhanced nanophotonic effects of nanowire (NW) mo
This master thesis presents the development of a vision-based feedback control system for automating sample placement in a virtual neutron beam emulated using a laser. The thesis was carried out on behalf of the European Spallation Source (ESS) to investigate the feasibility, possibilities and limitations of using a robotic automation system for sample alignment. Due to the radioactive research en
This thesis investigates the performance and feasibility of passive and active analog frequency compensation filters for audio signal processing in a distributed speaker system. One passive and one active circuit topology were investigated more thoroughly and then the active filter was realized on a PCB and evaluated with electrical, thermal and acoustic measurements. The work also resulted in tw
