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ClpP functions as the proteolytic subunit of the ATP-dependent Clp protease in eubacteria, mammals and plant chloroplasts. We have cloned a clpP gene, designated clpP1, from the cyanobacterium Synechococcus sp. PCC 7942. The monocistronic 591 bp gene codes for a protein 80% similar to one of four putative ClpP proteins in another cyanobacterium, Synechocystis sp. PCC 6803. The constitutive ClpP1 c

ClpP is the proteolytic subunit of the ATP-dependent Clp protease in eubacteria, mammals and plant chloroplasts. Cyanobacterial ClpP protein is encoded by a multigene family, producing up to four distinct isozymes. We have examined the importance of the first ClpP protein (ClpP1) isolated from the cyanobacterium Synechococcus sp. PCC 7942 for acclimation to ecologically relevant UV-B and low-tempe

Synechococcus sp. strain PCC 7942 has a second clpB gene that encodes a 97-kDa protein with novel features. ClpBII is the first ClpB not induced by heat shock or other stresses; it is instead an essential, constitutive protein. ClpBII is unable to complement ClpBI function for acquired thermotolerance. No truncated ClpBII version is normally produced, unlike other bacterial forms, while ectopic sy

In the cyanobacterium Synechococcus sp. strain PCC 7942 a multigene family of three different isozymes encodes the proteolytic subunit ClpP of the ATP-dependent Clp protease. In contrast to the monocistronic clpPI gene, clpPII and clpPIII are part of two bicistronic operons with clpX and clpR, respectively. Unlike most bacterial Clp proteins, the Synechococcus ClpP2, ClpP3, ClpR and ClpX proteins

The interactive effects of inorganic carbon status, temperature and light on chlorosis induced by nitrogen deficiency, and the roles of Clp proteases in this process were investigated. In wild-type cultures grown in high or ambient CO(2), following transfer to media lacking combined nitrogen, phycocyanin per cell dropped primarily through dilution of the pigment through cell division, and also suf

The gene encoding for amorpha-4,11-diene synthase from Artemisia annua was transformed into yeast Saccharomyces cerevisiae in two fundamentally different ways. First, the gene was subcloned into the galactose-inducible, high-copy number yeast expression vector pYeDP60 and used to transform the Saccharomyces cerevisiae strain CEN.PK113-5D. Secondly, amorpha-4,11-diene synthase gene, regulated by th

The xylem of gymnosperm trees consists mainly of tracheids, which generally have larger radial diameters and thinner walls in earlywood than in latewood. Information on possible chemical differences is limited, but according to a few studies, earlywood normally contains more lignin relative to cellulose than latewood. However, for methods giving results per gram dry matter, this result is expected

One of the many unresolved questions regarding the permeability of the glomerular filtration barrier is the reason behind the marked difference in permeability between albumin and polysaccharide probe molecules such as Ficoll and dextran of the same molecular size. Although the differences in permeability have been mainly attributed to charge effects, we have previously shown that this would requi

Background: Long-term use of opioid analgesics (OA) for chronic pain may result in opioid use disorder (OUD). This is associated with adverse outcomes for individuals, families and society. Treatment needs of people with OUD related to chronic pain are different compared to dependence related to use, and also injection, of illicit opioids. In Nordic countries, day-to-day practical advice to assist

Tundra regions are projected to warm rapidly during the coming decades. The tundra biome holds the largest terrestrial carbon pool, largely contained in frozen permafrost soils. With warming, these permafrost soils may thaw and become available for microbial decomposition, potentially providing a positive feedback to global warming. Warming may directly stimulate microbial metabolism but may also

Background: Knee arthroscopy is one of the most common surgical procedures worldwide and the number of arthroscopies has substantially increased in the last 30 years. Thus, our aim was to provide updated estimates on the risk of complications and compare it with the background risk in the general population. Methods: We identified patients aged 15-84 years with knee arthroscopy in the years 2005-2

The new sensing applications need enhanced computing capabilities to handle the requirements of complex and huge data processing. The Internet of Things (IoT) concept brings processing and communication features to devices. In addition, the Cloud Computing paradigm provides resources and infrastructures for performing the computations and outsourcing the work from the IoT devices. This scenario op

During a recent measurement campaign, we encountered some problems in extracting the rice factor from measurements. There were different answers to the question of what constitutes the correct line-of-sight component. This document highlights those issues and is meant to be a basis of discussion. Any feedback on the raised issues is welcome.

Underestimated feed force is a known systematic error in cutting simulations. It is considered a consequence of inaccurate friction models, but there are indicators that friction is not the only reason for the error. In some cases, the value of Coulomb friction must be over 1.0 to compensate for the feed force and such values cause over-estimated chip thickness for example. In turning, the ploughi

Background: We studied the impact of radical pericardiectomy on early and long-term patient survival, postoperative New York Heart Association (NYHA) functional class, and left ventricular ejection fraction in patients with chronic constrictive pericarditis compared to a sub-total pericardiectomy. Methods: From 1991 to 2016, 41 patients underwent pericardiectomy for chronic constrictive pericardit

The implementation of MIMO technology on compact mobile terminal devices poses a unique challenge for system designers. This is because it requires that multiple antennas be closely separated in a confined volume, which results in strong mutual coupling among the antennas and high spatial correlation for the signals. In this paper, we present a review on the latest developments of using uncoupled

Recent work on compact multiple antennas in a random environment is reviewed, including extensions of the energy density antenna and the MIMO cube. The performance of handsets with multiple antennas is dependent on many factors due to the close proximity of the antennas. The distribution of scatterers in the environment has an impact on the distributed directivity of the antennas, the powers absor

Conventionally, the spacing between adjacent elements in an antenna array are set at half-a-wavelength in order to obtain maximum spatial resolution while avoiding spurious sidelobes. However, for antenna arrays that are implemented in a compact manner, the spacing between the adjacent elements can be significantly less than half-a- wavelength. The small spacing between the antennas results in str

Recent theoretical and simulation studies reveal that closely coupled antennas with appropriately chosen impedance matching loads can yield desired characteristics of small antenna correlation coefficients and/or high received power levels. However, no experiment has been performed to verify these claims. Here, we describe an experimental setup used to investigate the correlation and received powe

Dansk udtale kan give selv den mest ihærdige svensker grå hår i hovedet. Tre relativt simple konstruktioner som ordet er dit, der er røg i køkkenet, og de skal nå toget til Odense er givetvis lette at forstå i en sms eller mail, fordi de fleste af ordene er genkendelige, måske bortset fra nå (hinna). Men optræder de tre konstruktioner i en samtale, er de hverken lette at forstå eller lette at udta