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The performance of a 5' nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The concentrations and interactions of the PCR reagents were evaluated on the basis of two detection responses, the threshold cycle (C(T)) and the fluorescence intensity by a normalized reporter value (DeltaR(n)). Th
Gene therapy holds great promise for treating many genetic diseases, including muscular dystrophies. Mutations in the gene encoding laminin alpha2 chain ? an extracellular protein prominently expressed in the neuromuscular system ? cause a severe neuromuscular disorder: congenital muscular dystrophy type 1A (MDC1A). Currently, there is no treatment for MDC1A. Preclinical studies are the first step
Children's utilization of curative care was studied to analyse the division of responsibilities between undifferentiated primary care and specialized care. All chronically ill (n = 510), a control group (n = 287) and the total population 0-15 years of age (n = 6080) in a primary care district were studied using register data. Chronically ill children comprised 8.4% of the total child population an
In this paper we present a novel method for predictive coding with application to transmission of speech over packet-switched networks. Our method uses multiplexing to distribute a part of the information about a segment of each speech signal in several data packets while keeping the data packet rate and payload for that part of the information unchanged. We investigate three multiplexing schemes:
In this paper, we address some Internet core network optimization issues related to a combination of Open Shortest Path First (OSPF) and Multi Protocol Label Switching (MPLS) routing protocols. Since MPLS can be combined with any routing protocol there has appeared an idea of coupling IP streams routed via the OSPF protocol and MPLS Label Switched Paths (LSPs) in the same network. One expected adv
Searches for resonant (ν) over tilde production in e(+)e(-) collisions under the assumption that R-parity is not conserved and that the dominant R-parity violating coupling is lambda(121) or lambda(131) used data recorded by DELPHI in 1997 to 2000 at centre-of-mass energies of 183 to 208 GeV. No deviation from the Standard Model was observed. Upper limits are given for the lambda(121) and lambda(1
Interactions between the cell cycle machinery and transcription factors play a central role in coordinating terminal differentiation and proliferation arrest. We here show that cyclin-dependent kinase 6 (Cdk6) is specifically expressed in proliferating hematopoietic progenitor cells, and that Cdk6 inhibits transcriptional activation by Runx1, but not C/ EBP alpha or PU. 1. Cdk6 inhibits Runx1 acti
The finite impulse response (FIR) function of the proposed filter is realised by integrating weighted signal currents in a given time window on a capacitor. The resulting capacitor voltage becomes the filter output, periodically available between integration and reset. In such an FIR filter, the hardware cost is delinked to the number of taps,
The bioelectrocatalytical reduction of H2O2 based on direct electron transfer (ET) between polycrystalline silver and the heme containing active site of horseradish peroxidase (HRP) is studied and compared with that obtained at gold. Native HRP and recombinant wild type HRP, containing additionally a six-histidine tag at the N-terminus, NHisrHRP, have been used for adsorptive modification of the e
The remarkable variation in plumage and morphological characters in the redpoll complex Carduelis flammea-hornemanni has puzzled taxonomists for several decades. In contrast, molecular studies have not revealed any genetic differentiation among the phenotypic redpoll forms. This could either be a result of high present-day gene flow or morphological differentiation following a rapid and recent pop
The additional transplantation of ex vivo generated hematopoietic (post)-progenitor cells represents a possible approach to ameliorate high-dose chemotherapy induced cytopenia. We investigated the feasibility of the large-scale expansion and transplantation of autologous megakaryocytic cells in four patients with advanced solid tumors. Up to 1,460x10(6) ex vivo generated cells were administered wi
In winter, small birds should be fat to avoid starvation and lean and agile to escape predators. This means that they face a tradeoff between the costs and benefits of carrying fat reserves. Every day they must gain enough fat to survive the coming night. Food-hoarding species can afford to carry less fat than nonhoarders because they can store energy outside the body. Furthermore, hoarders should
Temperature is an important factor regulating microbial activity and shaping the soil microbial community. Little is known, however, on how temperature affects the most important groups of the soil microorganisms, the bacteria and the fungi, in situ. We have therefore measured the instantaneous total activity (respiration rate), bacterial activity (growth rate as thymidine incorporation rate) and
High-resolution records of plant macrofossils, magnetic susceptibility, and total carbon content, complemented by pollen data, were obtained from a postglacial lake sediment sequence at alpine Lake Stentjarn (987 m a.s.l.), in west-central Sweden. Holocene vegetational and environmental changes were reconstructed from the data, with particular emphasis on the deglacial establishment of terrestrial
Studiens syfte var att undersöka hur många föräldrar som efter förlossningen önskade kontinuitet mellan graviditet och nyföddhetsperiod genom ett hembesök av den barnmorska som man lärt känna under graviditeten, hur dessa kvinnor och män formulerade sina erfarenheter och behov, om de skilde sig från föräldrar som inte önskade hembesök och om resultatet kunde ge synpunkter på en individualiserad hä
This thesis focuses on the provision and management of water supply and sanitation in housing areas in the City of Dar es Salaam. The main objective of the study is to contribute to knowledge and understanding of provision and management of urban water supply and sanitation in Tanzania in the context of sustainable urbanisation. A sectoral and single problem issue study approach was avoided and in
Adults normally perceive auditory scenes in terms of sound patterns emitted by concurrently active sources. Thus pattern formation is an important process of auditory object perception. The aim of the present study was to determine whether neonates group sounds by repeating pitch patterns. Standard ("S"; p = 80%) and deviant tones ("D", p = 20%) differing only in pitch were delivered either in a r
